APE1 Mouse Monoclonal Antibody [12H1]

About this product

The role of transcription factors in the regulation of gene expression is well established. Although the activity of these factors can be regulated by phosphorylation, evidence has indicated regulation of DNA binding mediated by changes in reduction-oxidation (redox) status. Mutational analysis has identified a single conserved cysteine residue mapping within the DNA binding domains of Fos and Jun. Chemical oxidation or modification of this cysteine residue inhibits the DNA binding activity of Fos and Jun. A similar mode of regulation has been recently proposed for other nuclear transcription factors. Oxidation is reversible by these compounds or by a cellular redox/DNA repair protein identified originally as Ref-1 (redox factor 1). Ref-1 is identical to a previously characterized DNA repair enzyme designated HAP1, APE or APEX.

Specifications

Catalog number

EM1801-11

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

AP endonuclease 1 antibody AP endonuclease class I antibody AP lyase antibody APE 1 antibody APE antibody APE-1 antibody APEN antibody APEX 1 antibody APEX antibody APEX nuclease (multifunctional DNA repair enzyme) 1 antibody Apex nuclease 1 antibody APEX nuclease antibody APEX1 antibody APEX1_HUMAN antibody Apurinic endonuclease antibody Apurinic-apyrimidinic endonuclease 1 antibody Apurinic/apyrimidinic (abasic) endonuclease antibody Apurinic/apyrimidinic endonuclease 1 antibody Apurinic/apyrimidinic exonuclease antibody APX antibody BAP1 antibody Deoxyribonuclease (apurinic or apyrimidinic) antibody DNA (apurinic or apyrimidinic site) lyase antibody DNA- (apurinic or apyrimidinic site) lyase, mitochondrial antibody EC 4.2.99.18 antibody HAP 1 antibody HAP1 antibody Human Apurinic endonuclease 1 antibody MGC139790 antibody Multifunctional DNA repair enzyme antibody Redox factor 1 antibody Redox factor-1 antibody REF 1 antibody REF 1 protein antibody REF-1 antibody REF1 antibody REF1 protein antibody

Abbreviation

APEN, AP endonuclease 1, APE-1, APEX1, APE, APE1, APEX, APX, HAP1, REF1

Swiss Prot

P27695 Human, P28352 Mouse, P43138 Rat

Cellular Locus

Nucleus. Endoplasmic reticulum, Cytoplasm.

Host

Mouse

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein within Human APE1 aa 20-318/318.

Isotype

IgG2a

Conjugation

Non-conjugated

Type

Mouse monoclonal Antibody

Applications

WB, IF-Cell, IHC-P, FC

Positive Control

HeLa cell lysate, LNCaP cell lysate, HEK-293 cell lysate, HL-60 cell lysate, A431 cell lysate, mouse brain tissue lysate, C6 cell lysate, rat testis tissue lysate, human kidney tissue, mouse liver tissue, rat colon tissue, SiHa.

Concentration

2 mg/mL

Dilution

WB: 1:1,000-1:5,000 ;IF-Cell: 1:50 ;IHC-P: 1:1,000 ;FC: 1:50-1:100

Purity

Immunogen affinity purified.

Form

Liquid

Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 36 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

No