Ribonuclease 3 Mouse Monoclonal Antibody [5G4]

About this product

The protein encoded by this gene belongs to the pancreatic ribonuclease family, a subset of the ribonuclease A superfamily. Cytotoxin and helminthotoxin with low-efficiency ribonuclease activity. The protein exhibits antimicrobial activity against pathogenic bacteria. Exhibits antibacterial activity, including cytoplasmic membrane depolarization of preferentially Gram-negative, but also Gram-positive strains. Promotes E.coli outer membrane detachment, alteration of the overall cell shape and partial loss of cell content. ECP is a potent cytotoxic protein capable of killing cells of guinea pig tracheal epithelium, mammalian leukemia, epidermis carcinoma, and breast carcinoma, as well as non-mammalian cells such as parasites, bacteria, and viruses. Mature ECP is cytotoxic to human bronchial epithelial (BEAS-2B) cells by specific binding to cell surface heparan sulfate proteoglycans (HSPGs) followed by endocytosis. ECP triggers apoptosis by caspase-8 activation through mitochondria-independent pathway. Increases in chromatin condensation, sub-G1 population, PARP cleavage, and DNA fragmentation indicate that ECP induces apoptosis in human bronchial epithelial (BEAS-2B) cells.

Specifications

Catalog number

EM1801-17

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

Yes

CAS Number

9007-83-4

Product Name Alternative

Cytotoxic ribonuclease antibody ECP antibody ECP_HUMAN antibody Eosinophil cationic protein antibody OTTHUMP00000164017 antibody Ribonuclease 3 antibody Ribonuclease, RNase A family, 3 antibody RNase 3 antibody RNASE3 antibody RNS3 antibody

Abbreviation

ECP, RNase 3

Swiss Prot

P12724 Human

Cellular Locus

Secreted.

Host

Mouse

Species Reactivity

Human

Immunogen

Recombinant protein within Human Ribonuclease 3 aa 8-140 / 160.

Isotype

IgG1

Conjugation

Non-conjugated

Type

Mouse monoclonal Antibody

Applications

WB, IF-Cell, IHC-P, FC

Positive Control

U937 cell lysate, Hela, SHG-44, human spleen tissue, human kidney tissue, Hela.

Concentration

2 mg/mL

Dilution

WB:1:500-1:2000 ;IF-Cell:1:100 ;IHC-P:1:50-1:200 ;FC:1:50-1:100

Purity

Protein G affinity purified.

Form

Liquid

Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 18 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

No