Hip1 Mouse Monoclonal Antibody [13E1]

About this product

Huntington disease is associated with the expansion of a polyglutamine tract, greater than 35 repeats, in the HD gene product huntingtin. HIP1 (huntingtin-interacting protein 1), a membrane-associated protein, binds specifically to the N-terminus of human huntingtin. HIP1 is ubiquitously expressed in different brain regions at low levels, and exhibits nearly identical subcellular fractionation as huntingtin. The huntingtin-HIP1 interaction is restricted to the brain and is inversely correlated to the polyglutamine length in the huntingtin, suggesting that loss of normal huntingtin-HIP1 interaction may compromise the membrane-cytoskeletal integrity in the brain. HIP1 contains an endocytic multidomain protein with a C-terminal Actin-binding domain, a central coiled-coil forming region and an N-terminal ENTH domain. HIP1 may be involved in vesicle trafficking; the structural integrity of HIP1 is crucial for maintenance of normal vesicle size in vivo. HIP12 is a non-proapoptotic member of the HIP gene family that is expressed in the brain and shares a similar subcellular distribution pattern with HIP1. However, HIP12 differs from HIP1 in its pattern of expression at both the mRNA and protein level. HIP12 does not directly interact with huntingtin but can interact with HIP1.

Specifications

Catalog number

EM1901-04

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

2610109B09Rik antibody A930014B11Rik antibody E130315I21Rik antibody HIP 1 antibody HIP I antibody HIP-1 antibody HIP-I antibody hip1 antibody HIP1/PDGFRB fusion gene antibody HIP1/PDGFRB fusion gene, included antibody HIP1_HUMAN antibody HIPI antibody Huntingtin interacting protein 1 antibody Huntingtin Interacting Protein HIP1 antibody Huntingtin-interacting protein 1 antibody Huntingtin-interacting protein I antibody ILWEQ antibody KIAA4113 antibody MGC126506 antibody MGC27616 antibody mKIAA4113 antibody

Abbreviation

HIP-1, HIP-I, Hip1, Kiaa4113

Swiss Prot

O00291 Human, Q8VD75 Mouse,

Cellular Locus

Nucleus. Cytoplasm.

Host

Mouse

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within Human Hip1 aa 1-50 / 1,029.

Isotype

IgG1

Conjugation

Non-conjugated

Type

Mouse monoclonal Antibody

Applications

WB, IF-Cell, IHC-P

Positive Control

HCT 116 cell lysates, SH-SY5Y, rat brain tissue, human colon cancer tissue, human prostate cancer tissue, mouse brain tissue.

Concentration

2 mg/mL

Dilution

WB: 1:500-1:2,000 ;IF-Cell: 1:50-1:200 ;IHC-P: 1:50-1:200

Purity

Immunogen affinity purified.

Form

Liquid

Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 116 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

No