MCM7 Mouse Monoclonal Antibody [15E1]

About this product

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage. Early fractionation of eukaryotic MCM proteins yielded a variety of dimeric, trimeric and tetrameric complexes with unclear biological significance. Specifically a MCM467 subcomplex is shown to have in vitro helicase activity which is inhibited by the MCM2 subunit. The MCM2-7 hexamer is the proposed physiological active complex.

Specifications

Catalog number

EM1901-12

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

CDABP0042 antibody CDC 47 antibody CDC47 antibody CDC47 homolog antibody Cdc47, S. cerevisiae, homolog of antibody DNA replication licensing factor MCM7 antibody Homolog of S. cerevisiae Cdc47 antibody MCM 2 antibody MCM 7 antibody MCM2 antibody MCM2, formerly antibody Mcm7 antibody MCM7 minichromosome maintenance deficient 7 antibody MCM7_HUMAN antibody Minichromosome Maintainence 7 antibody Minichromosome maintainence, S. cerevisiae, homolog of antibody Minichromosome maintenance complex component 7 antibody Minichromosome maintenance deficient 7 antibody Minichromosome maintenance protein 7 antibody P1.1 MCM3 antibody P1.1-MCM3 antibody P1CDC47 antibody P85MCM antibody PNAS 146 antibody PNAS146 antibody PPP1R104 antibody Protein phosphatase 1, regulatory subunit 104 antibody

Abbreviation

MCM7

Swiss Prot

P33993 Human, Q61881 Mouse,

Cellular Locus

Nucleus.

Host

Mouse

Species Reactivity

Human, Mouse, Rat, Monkey

Immunogen

Recombinant protein within Human MCM7 aa 516-719 / 719.

Isotype

IgG1

Conjugation

Non-conjugated

Type

Mouse monoclonal Antibody

Applications

WB, IHC-P, FC

Positive Control

HL-60 cell lysate, HEK-293 cell lysate, HeLa cell lysate, Jurkat cell lysate, SW480 cell lysate, LNCaP cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, PC-12 cell lysate, COS-1 cell lysate, human tonsil tissue, human esophagus tissue, SH-SY5Y.

Concentration

2 mg/mL

Dilution

WB:1:500-1:2,000 ; ICC/IHC-P:1:50-1:200 ; FC:1:50-1:100

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 81 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

No