ZAP70 Mouse Monoclonal Antibody [A1B5]

About this product

Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates motility, adhesion and cytokine expression of mature T-cells, as well as thymocyte development. Contributes also to the development and activation of primary B-lymphocytes. When antigen presenting cells (APC) activate T-cell receptor (TCR), a serie of phosphorylations lead to the recruitment of ZAP70 to the doubly phosphorylated TCR component CD247/CD3Z through ITAM motif at the plasma membrane. This recruitment serves to localization to the stimulated TCR and to relieve its autoinhibited conformation. Release of ZAP70 active conformation is further stabilized by phosphorylation mediated by LCK. In turn, a large number of signaling molecules are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation. Furthermore, ZAP70 controls cytoskeleton modifications, adhesion and mobility of T-lymphocytes, thus ensuring correct delivery of effectors to the APC. ZAP70 is also required for TCR-CD247/CD3Z internalization and degradation through interaction with the E3 ubiquitin-protein ligase CBL and adapter proteins SLA and SLA2. Thus, ZAP70 regulates both T-cell activation switch on and switch off by modulating TCR expression at the T-cell surface.

Specifications

Catalog number

EM1901-27

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

70 kDa zeta associated protein antibody 70 kDa zeta-associated protein antibody EC 2.7.10.2 antibody FLJ17670 antibody FLJ17679 antibody Selective T cell defect antibody SRK antibody STD antibody Syk related tyrosine kinase antibody Syk-related tyrosine kinase antibody Truncated ZAP kinase antibody Tyrosine protein kinase ZAP70 antibody Tyrosine-protein kinase ZAP-70 antibody TZK antibody ZAP 70 antibody ZAP70 antibody ZAP70_HUMAN antibody Zeta chain associated protein kinase 70kD antibody Zeta chain associated protein kinase 70kDa antibody Zeta chain associated protein kinase 70kDa isoform 1 antibody Zeta chain associated protein kinase 70kDa isoform 2 antibody Zeta chain of T cell receptor associated protein kinase 70 antibody Zeta chain TCR associated protein kinase 70kD antibody Zeta chain TCR associated protein kinase 70kDa antibody

Abbreviation

Tyrosine-protein kinase ZAP-70 70 kDa zeta-chain associated protein Syk-related tyrosine kinase ZAP70

Swiss Prot

P43403 Human, P43404 Mouse,

Cellular Locus

Cell membrane, cytoplasm.

Host

Mouse

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein within human ZAP70 aa 250-480.

Isotype

IgG2a

Conjugation

Non-conjugated

Type

Mouse monoclonal Antibody

Applications

WB, IHC-P, FC, IF-Cell

Positive Control

Jurkat cell lysate, mouse spleen tissue lysate, mouse thymus tissue lysate, rat spleen tissue lysate, rat thymus tissue lysate, human tonsil tissue, Jurakt.

Concentration

2 mg/mL

Dilution

WB: 1:1,000-1:2,000 ;IHC-P: 1:50-1:100 ;FC: 1:50-1:100 ;IF-Cell: 1:50

Purity

Protein G affinity purified.

Form

Liquid

Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 70 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

No