USP28 Mouse Monoclonal Antibody [A1F9]

Catalog number EM1901-46

Price for EM1901-46

Size / count 100 µL

About this product

Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN. Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting complex/cyclosome (APC/C). In contrast, it does not deubiquitinate PLK1. Specifically deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with isoform 1 of FBXW7 (FBW7alpha) in the nucleoplasm and counteracting ubiquitination of MYC by the SCF (FBW7) complex. In contrast, it does not interact with isoform 4 of FBXW7 (FBW7gamma) in the nucleolus, allowing MYC degradation and explaining the selective MYC degradation in the nucleolus. Deubiquitinates ZNF304, hence preventing ZNF304 degradation by the proteasome and leading to the activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) in a subset of colorectal cancers (CRC) cells.

Specifications

Catalog number: EM1901-46
Size / count: 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Product Name Alternative: Deubiquitinating enzyme 28 antibody KIAA1515 antibody Ubiquitin carboxyl terminal hydrolase 28 antibody Ubiquitin carboxyl-terminal hydrolase 28 antibody ubiquitin carboxyl-terminal hydrolase 28 variant 1 antibody Ubiquitin specific peptidase 28 antibody Ubiquitin specific processing protease 28 antibody Ubiquitin specific protease 28 antibody Ubiquitin thioesterase 28 antibody Ubiquitin thiolesterase 28 antibody Ubiquitin-specific-processing protease 28 antibody UBP28_HUMAN antibody USP 28 antibody USP28 antibody USP28 protein antibody
Abbreviation: USP28
Swiss Prot: Q96RU2 Human, D3ZJ96 Rat
Cellular Locus: Nucleoplasm.
Host: Mouse
Species Reactivity: Human, Rat
Immunogen: Recombinant protein within Human USP28 aa 509-860 / 1,077.
Isotype: IgG1
Conjugation: Non-conjugated
Type: Mouse monoclonal Antibody
Applications: WB
Positive Control: HeLa cell lysate, A431 cell lysate, 293T cell lysate, SW480 cell lysate, LNCaP cell lysate, MDA-MB-231 cell lysate, MDA-MB-468 cell lysate, rat testis tissue lysate.
Concentration: 2 mg/mL
Dilution: WB:1:500-1:2,000
Purity: Protein G affinity purified.
Form: Liquid
Buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight: Predicted band size: 122 kDa
Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody: No

Frequently Asked Questions

What is the catalog number for USP28 Mouse Monoclonal Antibody [A1F9]?

The catalog number for USP28 Mouse Monoclonal Antibody [A1F9] is EM1901-46.

What sizes are available for USP28 Mouse Monoclonal Antibody [A1F9]?

USP28 Mouse Monoclonal Antibody [A1F9] is available in the following size(s): 100 µL.

Is USP28 Mouse Monoclonal Antibody [A1F9] in stock?

USP28 Mouse Monoclonal Antibody [A1F9] is currently made to order / not held in immediate stock. Contact us for lead time.

What is USP28 Mouse Monoclonal Antibody [A1F9] used for?

Is this product tested for endotoxin levels?

Endotoxin testing status is noted on the datasheet where applicable, particularly for recombinant proteins intended for cell-based or functional assays. If this isn’t listed and you need confirmation, contact support with the catalogue number.

Can I request additional validation data, such as western blot images, for this product?

Additional data may be available on request. Contact our technical support team with the catalogue number and we’ll check what supporting documentation the supplier can provide.

What documentation should I request if this product is used for a regulated or intellectual-property-sensitive application?

If the product is subject to intellectual property protections, we’ll advise on any MTA or usage restrictions before shipping. Let us know your intended end use so we can confirm what’s required upfront.