ERK2 Mouse Monoclonal Antibody [C3B12]

About this product

Mitogen-activated protein kinase (MAPK) signaling pathways involve two closely related MAP kinases, known as extracellular-signal-related kinase 1 (ERK 1, p44) and 2 (ERK 2, p42). Growth factors, steroid hormones, G protein-coupled receptor ligands, and neurotransmitters can initiate MAPK signaling pathways. Activation of ERK1 and ERK2 requires phosphorylation by upstream kinases such as MAP kinase kinase (MEK), MEK kinase and Raf-1. ERK1 and ERK2 phosphorylation can occur at specific tyrosine and threonine sites mapping within consensus motifs that include the Threonine-Glutamate-Tyrosine motif. ERK activation leads to dimerization with other ERKs and subsequent localization to the nucleus. Active ERK dimers phosphorylate serine and threonine residues on nuclear proteins and influence a host of responses that include proliferation, differentiation, transcription regulation and development. The human ERK2 gene maps to chromosome 22q11.21 and encodes a 360-amino acid protein.

Specifications

Catalog number

EM1901-55

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

ERK 2 antibody ERK-2 antibody ERT1 antibody Extracellular Signal Regulated Kinase 2 antibody Extracellular signal-regulated kinase 2 antibody MAP kinase 1 antibody MAP kinase 2 antibody MAP kinase isoform p42 antibody MAPK 1 antibody MAPK 2 antibody Mapk1 antibody MAPK2 antibody Mitogen-activated protein kinase 1 antibody Mitogen-activated protein kinase 2 antibody MK01_HUMAN antibody P38 antibody P40 antibody P41 antibody p42-MAPK antibody P42MAPK antibody PRKM1 antibody PRKM2 antibody protein kinase, mitogen-activated, 1 antibody protein kinase, mitogen-activated, 2 antibody protein tyrosine kinase ERK2 antibody

Abbreviation

MAPK1, ERK2, PRKM1, PRKM2

Swiss Prot

P28482 Human, P63085 Mouse, P63086 Rat

Cellular Locus

Cytoplasm, cytoskeleton, spindle, Nucleus, microtubule organizing center, centrosome, Membrane, caveola, Cell junction, focal adhesion.

Host

Mouse

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein within human ERK2 aa 200-360.

Isotype

IgG1

Conjugation

Non-conjugated

Type

Mouse monoclonal Antibody

Applications

WB, IHC-P, FC

Positive Control

HeLa cell lysate, Jurkat cell lysate, A549 cell lysate, A431 cell lysate, HepG2 cell lysate, HEK-293 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, C6 cell lysate, PC-12 cell lysate, human brain tissue lysate, mouse brain tissue lysate, rat brain tissue lysate, human thyroid tissue, human skin tissue, human breast carcinoma tissue, human pancreas tissue, mouse testis tissue, mouse colon tissue, mouse ovary tissue, K562.

Concentration

2 mg/mL

Dilution

WB: 1:3,000 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100

Purity

Protein G affinity purified.

Form

Liquid

Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 41 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

No