GAPDH Mouse Monoclonal Antibody [12D7]

About this product

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.

Specifications

Catalog number

EM1901-57

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

38 kDa BFA-dependent ADP-ribosylation substrate antibody aging associated gene 9 protein antibody Aging-associated gene 9 protein antibody BARS-38 antibody cb609 antibody EC 1.2.1.12 antibody Epididymis secretory sperm binding protein Li 162eP antibody G3P_HUMAN antibody G3PD antibody G3PDH antibody GAPD antibody GAPDH antibody Glyceraldehyde 3 phosphate dehydrogenase antibody glyceraldehyde 3-PDH antibody Glyceraldehyde-3-phosphate dehydrogenase antibody HEL-S-162eP antibody KNC-NDS6 antibody MGC102544 antibody MGC102546 antibody MGC103190 antibody MGC103191 antibody MGC105239 antibody MGC127711 antibody MGC88685 antibody OCAS, p38 component antibody OCT1 coactivator in S phase, 38-KD component antibody peptidyl cysteine S nitrosylase GAPDH antibody Peptidyl-cysteine S-nitrosylase GAPDH antibody wu:fb33a10 antibody

Abbreviation

GAPDH

Swiss Prot

P04406 Human, P16858 Mouse, P04797 Rat

Cellular Locus

Cytoskeleton, nucleus, cytosol, perinuclear region, membrane.

Host

Mouse

Species Reactivity

Human, Mouse, Rat, Zebrafish

Immunogen

Synthetic peptide within Human GAPDH aa 174-223 / 335.

Isotype

IgG1

Conjugation

Non-conjugated

Type

Mouse monoclonal Antibody

Applications

WB, IHC-P, FC

Positive Control

HepG2 cell lysate, PC-12 cell lysate, F9 cell lysate, A549 cell lysate, human tonsil tissue, human colon carcinoma tissue, human esophagus tissue, human small intestine tissue, human pancreas tissue, A549.

Concentration

2 mg/mL

Dilution

WB:1:1,000-1:5,000 ;IHC-P:1:50-1:100 ;FC:1:50-1:100

Purity

Protein G affinity purified.

Form

Liquid

Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 36 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

No