LYRIC Mouse Monoclonal Antibody [C6-C10]

About this product

Metadherin, also known as protein LYRIC or astrocyte elevated gene-1 protein (AEG-1) is a protein that in humans is encoded by the MTDH gene. MTDH (AEG-1) is involved in HIF-1alpha mediated angiogenesis. MTDH also interacts with SND1 and involved in RNA-induced silencing complex (RISC) and plays very important role in RISC and miRNA functions. MTDH has been shown to interact with spliceosome proteins in the cell nucleus and regulate the process of alternative splicing. MTDH induces an oncogene called Late SV40 factor (LSF/TFCP2) which is involved in thymidylate synthase (TS) induction and DNA biosynthesis synthesis. Late SV40 factor (LSF/TFCP2) enhances angiogenesis by transcriptionally up-regulating matrix metalloproteinase-9 (MMP9). MTDH acts as an oncogene in melanoma, malignant glioma, breast cancer and hepatocellular carcinoma. It is highly expressed in these cancers and helps in progression and development of these cancers. It is induced by c-Myc oncogene and plays very important role in anchorage independent growth of cancer cells. Elevated expression of the metastasis gene metadherin (MTDH), which is overexpressed in more than 40% of breast cancers, is associated with poor clinical outcomes.

Specifications

Catalog number

EM1901-60

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

3D3 antibody 3D3/LYRIC antibody AEG 1 antibody AEG-1 antibody AEG1 antibody Astrocyte elevated gene 1 antibody Astrocyte elevated gene-1 protein antibody LYRIC antibody LYRIC/3D3 antibody LYRIC_HUMAN antibody Lysine rich CEACAM1 associated protein antibody Lysine rich CEACAM1 co isolated protein antibody Lysine-rich CEACAM1 co-isolated protein antibody Metadherin antibody Metastasis adhesion protein antibody MTDH antibody Protein LYRIC antibody

Abbreviation

MTDH

Swiss Prot

Q86UE4 Human, Q80WJ7 Mouse, Q9Z1W6 Rat

Cellular Locus

Nucleus membrane, nucleolus, endoplasmic reticulum membrane, tight junction, perinuclear region.

Host

Mouse

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein within Human LYRIC aa 6-233 / 582.

Isotype

IgG1

Conjugation

Non-conjugated

Type

Mouse monoclonal Antibody

Applications

WB, IHC-P, FC, IF-Cell

Positive Control

MCF-7 cell lysate, Jurkat cell lysate, rat testis tissue, human breast carcinoma tissue, mouse brain tissue, K562, Jurkat.

Concentration

2 mg/mL

Dilution

WB: 1:500-1:2,000 ;IHC-P: 1:200-1:1,000 ;FC: 1:50-1:100 ;IF-Cell: 1:100

Purity

Protein G affinity purified.

Form

Liquid

Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size 64 kDa.

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

No