UBA3 Mouse Monoclonal Antibody [1B6-6-5]

About this product

NEDD8-activating enzyme E1 catalytic subunit is a protein that in humans is encoded by the UBA3 gene. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme associates with AppBp1, an amyloid beta precursor protein binding protein, to form a heterodimer, and then the enzyme complex activates NEDD8, a ubiquitin-like protein, which regulates cell division, signaling and embryogenesis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. This enzyme contains an E2 binding domain, which resembles ubiquitin, and recruits the catalytic core of the E2 enzyme UBE2M (Ubc12) in a similar manner to that in which ubiquitin interacts with ubiquitin binding domains.

Specifications

Catalog number

EM1901-64

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

DKFZp566J164 antibody EC 6.3.2. antibody hUba3 antibody MGC22384 antibody NEDD8 activating enzyme E1 catalytic subunit antibody NEDD8 activating enzyme E1C antibody Nedd8 activating enzyme hUba3 antibody NEDD8-activating enzyme E1 catalytic subunit antibody NEDD8-activating enzyme E1C antibody uba3 antibody UBA3 ubiquitin activating enzyme E1 homolog antibody UBA3_HUMAN antibody UBE1C antibody Ubiquitin activating enzyme 3 antibody Ubiquitin activating enzyme E1C antibody Ubiquitin-activating enzyme 3 antibody Ubiquitin-activating enzyme E1C antibody Ubiquitin-like modifier-activating enzyme 3 antibody

Abbreviation

UBA3

Swiss Prot

Q8TBC4 Human

Cellular Locus

Cytoplasm, cytosol, nucleus.

Host

Mouse

Species Reactivity

Human, Rat

Immunogen

Recombinant protein within Human UBA3 aa 220-424 / 463.

Isotype

IgG1

Conjugation

Non-conjugated

Type

Mouse monoclonal Antibody

Applications

WB, IHC-P, FC

Positive Control

293T cell lysates, 293 cell lysate, HL-60 cell lysate, human lung tissue, human lung carcinoma tissue, human breast carcinoma tissue, SiHa.

Concentration

2 mg/mL

Dilution

WB:1:500-1:2,000 ;IHC-P:1:50-1:200 ;FC:1:50-1:100

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 52 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

No