ACY1 Mouse Monoclonal Antibody [15G2]

About this product

Aminoacylase is involved in the regulation of the urea cycle. N-acetyl-L-glutamate is an allosteric activator of carbamoyl phosphate synthetase, a crucial enzyme that commits NH4+ molecules to the urea cycle. The urea cycle gets rid of excess ammonia (NH4+) in the body, a process that must be up-regulated during times of increased protein catabolism, as amino acid breakdown produces large amounts of NH4+. When amino acid catabolism increases, N-Acetylglutamate synthase is up-regulated, producing more N-acetyl-L-glutamate, which up-regulates carbamoyl phosphate synthetase and allows it to dispose of the excess NH4+ from catabolism.Aminoacylase is up-regulated during times of nutrient deficit or starvation, causing N-acetyl-L-glutamate breakdown, which down-regulates carbamoyl phosphate synthetase and the rest of the urea cycle. This response is evolutionarily advantageous, since a nutrient deficit means there isn't as much NH4+ that needs to be disposed of and since the body wants to salvage as many amino acids as it can.

Specifications

Catalog number

EM1901-86

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

ACY 1 antibody ACY-1 antibody Acy1 antibody ACY1_HUMAN antibody ACY1D antibody ACYLASE antibody Acylase I antibody Aminoacylase 1 antibody Aminoacylase-1 antibody EC 3.5.1.14 antibody epididymis secretory protein Li 5 antibody HEL-S-5 antibody N acyl L amino acid amidohydrolase antibody N-acyl-L-amino-acid amidohydrolase antibody OTTHUMP00000212459 antibody OTTHUMP00000212462 antibody OTTHUMP00000212463 antibody OTTHUMP00000212464 antibody OTTHUMP00000212465 antibody

Abbreviation

ACY-1,Aminoacylase-1,N-acyl-L-amino-acid amidohydrolase,ACY1

Swiss Prot

Q03154 Human, Q99JW2 Mouse, Q6AYS7 Rat

Cellular Locus

Cytoplasm.

Host

Mouse

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein within Human ACY1 aa 55-256 / 408.

Isotype

IgG2a

Conjugation

Non-conjugated

Type

Mouse monoclonal Antibody

Applications

WB, IHC-P, FC

Positive Control

K-562 cell lysate, HepG2 cell lysate, PC-3M cell lysate, human liver tissue lysate, human kidney tissue lysate, mouse liver tissue lysate, mouse kidney tissue lysate, rat liver tissue lysate, rat kidney tissue lysate, human kidney tissue, mouse kidney tissue, rat kidney tissue, SHSY5Y.

Concentration

2 mg/mL

Dilution

WB: 1:500-1:1,000 ;IHC-P: 1:2,000 ;FC: 1:50-1:100

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 46 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

No