CD163 Mouse Monoclonal Antibody [A3B3]

About this product

Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions. Intravenous lipopolysaccharide (LPS) produces a rapid rise of sCD163 in plasma of patient as it induces metalloproteinase-mediated shedding from monocytes surface. The soluble form (sCD163) in plasma is a novel parameter in diseases affecting macrophage function and monocyte/macrophage load in the body. The concentration of sCD163 is probably reflecting the number of macrophages of the 'alternative macrophage activation' phenotype with a high CD163 expression playing a major role in dampening the inflammatory response and scavenging components of damaged cells. This has initiated a number of clinical studies for evaluation of sCD163 as a disease marker in inflammatory conditions e.g. infection, autoimmune disease, transplantation, atherosclerosis and cancer.

Specifications

Catalog number

EM1901-90

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

C163A_HUMAN antibody CD 163 antibody CD163 antibody CD163 antigen antibody CD163 molecule antibody Hemoglobin scavenger receptor antibody M130 antibody M130 antigen precursor antibody Macrophage associated antigen antibody MM130 antibody OTTHUMP00000238617 antibody OTTHUMP00000238618 antibody OTTHUMP00000238619 antibody OTTHUMP00000238620 antibody SCARI1 antibody Scavenger receptor cysteine rich type 1 protein M130 antibody sCD163 antibody Soluble CD163 antibody

Abbreviation

CD163

Swiss Prot

Q86VB7 Human

Cellular Locus

Secreted, cell membrane.

Host

Mouse

Species Reactivity

Human

Immunogen

Recombinant protein within human CD163 aa 1-170.

Isotype

IgG1

Conjugation

Non-conjugated

Type

Mouse monoclonal Antibody

Applications

WB, IHC-P, FC

Positive Control

Human lung tissue lysate, human liver tissue lysate, human lung tissue, human liver carcinoma tissue, human colom tissue, human placenta tissue, HT-29.

Concentration

2 mg/mL

Dilution

WB:1:500-1:2,000 ;IHC-P:1:100-1:500 ;FC:1:50-1:100

Purity

Protein G affinity purified.

Form

Liquid

Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 125 kDa.

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

No