HMGB2 Mouse Monoclonal Antibody [10D1]

About this product

Multifunctional protein with various roles in different cellular compartments. May act in a redox sensitive manner. In the nucleus is an abundant chromatin-associated non-histone protein involved in transcription, chromatin remodeling and V (D)J recombination and probably other processes. Binds DNA with a preference to non-canonical DNA structures such as single-stranded DNA. Can bent DNA and enhance DNA flexibility by looping thus providing a mechanism to promote activities on various gene promoters by enhancing transcription factor binding and/or bringing distant regulatory sequences into close proximity. Involved in V (D)J recombination by acting as a cofactor of the RAG complex: acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Proposed to be involved in the innate immune response to nucleic acids by acting as a promiscuous immunogenic DNA/RNA sensor which cooperates with subsequent discriminative sensing by specific pattern recognition receptors. In the extracellular compartment acts as a chemokine. Promotes proliferation and migration of endothelial cells implicating AGER/RAGE. Has antimicrobial activity in gastrointestinal epithelial tissues. Involved in inflammatory response to antigenic stimulus coupled with proinflammatory activity. Involved in modulation of neurogenesis probably by regulation of neural stem proliferation. Involved in articular cartilage surface maintenance implicating LEF1 and the Wnt/beta-catenin pathway.

Specifications

Catalog number

EM1902-06

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

C80539 antibody High mobility group (nonhistone chromosomal) protein 2 antibody High mobility group box 2 antibody High mobility group protein 2 antibody High mobility group protein B2 antibody HMG 2 antibody HMG B2 antibody HMG-2 antibody HMG2 antibody HMGB2 antibody HMGB2_HUMAN antibody

Abbreviation

HMGB2

Swiss Prot

P26583 Human, P30681 Mouse, P52925 Rat

Cellular Locus

Nucleus, secreted, chromosome, cytoplasm.

Host

Mouse

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein within Human HMGB2 aa 1-209 / 209.

Isotype

IgG2b

Conjugation

Non-conjugated

Type

Mouse monoclonal Antibody

Applications

WB, IHC-P

Positive Control

HeLa cell lysate, K-562 cell lysate, 293T cell lysate, RAW264.7 cell lysate, PC-12 cell lysate, human tonsil tissue, human cervical tissue, human thyroid tissue, human colon carcinoma tissue, human spleen tissue, human prostate carcinoma tissue, human breast carcinoma tissue, mouse liver tissue, mouse testis tissue, mouse fallopian tube tissue, mouse small intestine tissue.

Concentration

2 mg/mL

Dilution

WB: 1:500-1:1,000 ;IHC-P: 1:200-1:1,000

Purity

Protein G affinity purified.

Form

Liquid

Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 24 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

No