CD22 Mouse Monoclonal Antibody [A2G6]

About this product

CD22, or cluster of differentiation-22, is a molecule belonging to the SIGLEC family of lectins. It is found on the surface of mature B cells and to a lesser extent on some immature B cells. Generally speaking, CD22 is a regulatory molecule that prevents the overactivation of the immune system and the development of autoimmune diseases. CD22 is a sugar binding transmembrane protein, which specifically binds sialic acid with an immunoglobulin (Ig) domain located at its N-terminus. The presence of Ig domains makes CD22 a member of the immunoglobulin superfamily. CD22 functions as an inhibitory receptor for B cell receptor (BCR) signaling. Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules.

Specifications

Catalog number

EM1902-13

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

B cell receptor CD22 precursor antibody B lymphocyte cell adhesion molecule antibody B-cell receptor CD22 antibody B-lymphocyte cell adhesion molecule antibody BL CAM antibody BL-CAM antibody BLCAM antibody CD 22 antibody CD22 antibody CD22 antigen antibody CD22 molecule antibody CD22 protein antibody CD22_HUMAN antibody Lectin 2 antibody Leu14 antibody Lyb8 antibody MGC130020 antibody sialic acid binding Ig like lectin 2 antibody Sialic acid binding immunoglobulin like lectin 2 antibody Sialic acid-binding Ig-like lectin 2 antibody SIGLEC 2 antibody Siglec-2 antibody SIGLEC2 antibody T cell surface antigen Leu 14 antibody T-cell surface antigen Leu-14 antibody

Abbreviation

B-cell receptor CD22 B-lymphocyte cell adhesion molecule BL-CAM Siglec-2 CD22 SIGLEC2

Swiss Prot

P20273 Human

Cellular Locus

Cell membrane.

Host

Mouse

Species Reactivity

Human

Immunogen

Recombinant protein within Human CD22 aa 20-200.

Isotype

IgG1

Conjugation

Non-conjugated

Type

Mouse monoclonal Antibody

Applications

WB, IHC-P, FC

Positive Control

Raji cell lysate, Daudi cell lysate, human spleen tissue, Daudi.

Concentration

2 mg/mL

Dilution

WB:1:500-1:1,000 ;IHC-P:1:50-1:200 ;FC:1:50-1:100

Purity

Protein G affinity purified.

Form

Liquid

Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 95 kDa.

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

No