NuMA Mouse Monoclonal Antibody [A3A2]

About this product

Microtubule (MT)-binding protein that plays a role in the formation and maintenance of the spindle poles and the alignement and the segregation of chromosomes during mitotic cell division. Functions to tether the minus ends of MTs at the spindle poles, which is critical for the establishment and maintenance of the spindle poles. Plays a role in the establishment of the mitotic spindle orientation during metaphase and elongation during anaphase in a dynein-dynactin-dependent manner. In metaphase, part of a ternary complex composed of GPSM2 and G (i) alpha proteins, that regulates the recruitment and anchorage of the dynein-dynactin complex in the mitotic cell cortex regions situated above the two spindle poles, and hence regulates the correct oritentation of the mitotic spindle. During anaphase, mediates the recruitment and accumulation of the dynein-dynactin complex at the cell membrane of the polar cortical region through direct association with phosphatidylinositol 4,5-bisphosphate (PI (4,5)P2), and hence participates in the regulation of the spindle elongation and chromosome segregation. Also required for proper orientation of the mitotic spindle during asymmetric cell divisions. Plays a role in mitotic MT aster assembly. Positively regulates TNKS protein localization to spindle poles in mitosis.

Specifications

Catalog number

EM1902-16

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

Centrophilin stabilizes mitotic spindle in mitotic cells antibody NMP 22 antibody Nuclear matrix protein 22 antibody Nuclear mitotic apparatus protein 1 antibody Nuclear mitotic apparatus protein antibody NUMA 1 antibody NUMA antibody NuMA protein antibody NUMA1 antibody NUMA1_HUMAN antibody SP H antigen antibody SP-H antigen antibody Structural nuclear protein antibody

Abbreviation

Nuclear mitotic apparatus protein 1 Nuclear matrix protein-22 Nuclear mitotic apparatus protein SP-H antigen NUMA1 NMP22 NUMA

Swiss Prot

Q14980 Human

Cellular Locus

Nucleus, Cytoskeleton, Plasma membrane.

Host

Mouse

Species Reactivity

Human

Immunogen

Recombinant protein within Human NuMA aa 1-200 / 2,115.

Isotype

IgG1

Conjugation

Non-conjugated

Type

Mouse monoclonal Antibody

Applications

WB, IF-Cell, IHC-P, FC

Positive Control

Raji cell lysates, MCF-7, human tonsil tissue, human lung tissue, human cervix tissue, human thyroid tissue, human colon carcinoma tissue, human skin tissue, human prostate carcinoma tissue, human breast carcinoma tissue, human stomach carcinoma tissue, human small intestine tissue, human pancreas tissue, SH-SY5Y.

Concentration

2 mg/mL

Dilution

WB:1:500-1:2,000 ;IF-Cell:1:50-1:100 ;IHC-P:1:500-1:2,000 ;FC:1:50-1:100

Purity

Protein G affinity purified.

Form

Liquid

Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 238 kDa kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

No