SARS-CoV-2 Nucleocapsid Protein Mouse Monoclonal Antibody [A4D12]

About this product

Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.

Specifications

Catalog number

EM1902-21

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

SARS-CoV-2 Nucleocapsid Protein antibody SARS-CoV-2 NP antibody nucleocapsid protein antibody novel coronavirus N Protein antibody novel coronavirus Nucleocapsid Protein antibody 2019-nCoV Nucleoprotein antibody 2019-nCoV N antibody 2019nCoV N antibody 2019-nCoV N Protein antibody 2019 peak N Protein antibody 2019-nCoV nucleocapsid protein antibody

Abbreviation

SARS-CoV-2 Nucleocapsid Protein SARS-CoV-2 NP nucleocapsid protein novel coronavirus N Protein novel coronavirus Nucleocapsid Protein 2019-nCoV Nucleoprotein 2019-nCoV N 2019nCoV N 2019-nCoV N Protein 2019 peak N Protein 2019-nCoV nucleocapsid protein

Swiss Prot

P0DTC9

Cellular Locus

Virion, Host endoplasmic reticulum-Golgi intermediate compartment, Host Golgi apparatus.

Host

Mouse

Species Reactivity

SARS-CoV-2

Immunogen

Recombinant SARS-CoV-2 Nucleocapsid Protein.

Isotype

IgG1

Conjugation

Non-conjugated

Type

Mouse monoclonal Antibody

Applications

ELISA, WB

Positive Control

ELISA: Recombinant SARS-CoV-2 Nucleocapsid Protein. WB: N-terminal His tag-tagged SARS-CoV-2 Nucleocapsid protein and inactivated native SARS-CoV-2 lysate.

Concentration

2 mg/mL

Dilution

ELISA: Use at an assay dependent concentration. ;WB: 1:500-1:5,000

Purity

Protein G affinity purified.

Form

Liquid

Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

46 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

No