DAP Kinase 1 Mouse Monoclonal Antibody [12B1] — image 1 of 3

Product details

DAP Kinase 1 Mouse Monoclonal Antibody [12B1]

Catalog number EM1902-25

Price for EM1902-25

Size / count 100 µL

About this product

Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. Regulates both type I apoptotic and type II autophagic cell deaths signal, depending on the cellular setting. The former is caspase-dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Phosphorylates PIN1 resulting in inhibition of its catalytic activity, nuclear localization, and cellular function. Phosphorylates TPM1, enhancing stress fiber formation in endothelial cells. Phosphorylates STX1A and significantly decreases its binding to STXBP1. Phosphorylates PRKD1 and regulates JNK signaling by binding and activating PRKD1 under oxidative stress. Phosphorylates BECN1, reducing its interaction with BCL2 and BCL2L1 and promoting the induction of autophagy. Phosphorylates TSC2, disrupting the TSC1-TSC2 complex and stimulating mTORC1 activity in a growth factor-dependent pathway. Phosphorylates RPS6, MYL9 and DAPK3. Acts as a signaling amplifier of NMDA receptors at extrasynaptic sites for mediating brain damage in stroke. Cerebral ischemia recruits DAPK1 into the NMDA receptor complex and it phosphorylates GRINB at Ser-1303 inducing injurious Ca2+ influx through NMDA receptor channels, resulting in an irreversible neuronal death. Required together with DAPK3 for phosphorylation of RPL13A upon interferon-gamma activation which is causing RPL13A involvement in transcript-selective translation inhibition.

Specifications

Catalog number: EM1902-25
Size / count: 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Product Name Alternative: DAK1 antibody DAP K1 antibody DAP kinase 1 antibody DAPK 1 antibody DAPK antibody DAPK1 antibody DAPK1_HUMAN antibody Death Associated Protein Kinase 1 antibody Death-associated protein kinase 1 antibody DKFZp781I035 antibody
Abbreviation: DAP kinase 1,DAPK1,Death-associated protein kinase 1
Swiss Prot: P53355 Human, Q80YE7 Mouse
Cellular Locus: Cytoplasm, Cytoskeleton.
Host: Mouse
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant protein within Human DAPK1 aa 447-683 / 1,430.
Isotype: IgG1
Conjugation: Non-conjugated
Type: Mouse monoclonal Antibody
Applications: WB, IHC-P, IF-Cell
Positive Control: Human skin tissue lysate, human skeletal muscle tissue lysate, MCF-7, rat lung tissue, human lung cancer tissue, mouse liver tissue, LOVO.
Concentration: 2mg/mL
Dilution: WB: 1:500-1:2,000 ;IHC-P: 1:50-1:200 ;IF-Cell: 1:50-1:100
Purity: Protein A affinity purified.
Form: Liquid
Buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight: Predicted band size: 160 kDa
Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody: No

Frequently Asked Questions

What is the catalog number for DAP Kinase 1 Mouse Monoclonal Antibody [12B1]?

The catalog number for DAP Kinase 1 Mouse Monoclonal Antibody [12B1] is EM1902-25.

What sizes are available for DAP Kinase 1 Mouse Monoclonal Antibody [12B1]?

DAP Kinase 1 Mouse Monoclonal Antibody [12B1] is available in the following size(s): 100 µL.

Is DAP Kinase 1 Mouse Monoclonal Antibody [12B1] in stock?

DAP Kinase 1 Mouse Monoclonal Antibody [12B1] is currently made to order / not held in immediate stock. Contact us for lead time.

What is DAP Kinase 1 Mouse Monoclonal Antibody [12B1] used for?

What should I check when this product first arrives?

Inspect the package immediately for transportation damage, ice loss, leakage, or breakage. Report any issues right away with photos and your order reference; our specialists will determine whether the product is still usable or needs replacing.

Does this cell line or biologic require a Material Transfer Agreement (MTA)?

Some products, including certain cell lines, plasmids, and tissue cultures, are subject to intellectual property terms and may require an MTA before we can ship. We’ll inform you in advance if this product requires one, and additional handling fees may apply.

What should I do if I need to report a performance issue with a specific lot of this product?

Provide the catalogue number, product name, lot number, and your experimental results to our technical support team. They’ll investigate and, if needed, escalate to the manufacturer, provided the product was used before expiry and stored correctly.