GADD34 Mouse Monoclonal Antibody [A4D7]

About this product

Protein phosphatase 1 regulatory subunit 15A also known as growth arrest and DNA damage-inducible protein GADD34 is a protein that in humans is encoded by the PPP1R15A gene. The Gadd34/MyD116 gene was originally discovered as a member in a set of gadd and MyD mammalian genes encoding acidic proteins that synergistically suppress cell growth. Later on it has been characterized as a gene playing a role in ER stress-induced cell death, being a target of ATF4 that plays a role in ER-mediated cell death via promoting protein dephosphorylation of eIF2α and reversing translational inhibition. This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The induction of this gene by ionizing radiation occurs in certain cell lines regardless of p53 status, and its protein response is correlated with apoptosis following ionizing radiation.

Specifications

Catalog number

EM1902-26

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

Apoptosis associated protein antibody GADD 34 antibody Growth arrest and DNA damage inducible 34 antibody Growth arrest and DNA damage-inducible protein GADD34 antibody Myeloid differentiation primary response protein MyD116 homolog antibody Ppp1r15a antibody PR15A_HUMAN antibody Protein phosphatase 1 regulatory (inhibitor) subunit 15A antibody Protein phosphatase 1 regulatory subunit 15A antibody

Abbreviation

PPP1R15A

Swiss Prot

O75807 Human

Cellular Locus

Endoplasmic reticulum membrane, Mitochondrion outer membrane.

Host

Mouse

Species Reactivity

Human

Immunogen

Synthetic peptide within Human GADD34 aa 1-50 / 674.

Isotype

IgG1

Conjugation

Non-conjugated

Type

Mouse monoclonal Antibody

Applications

WB, IHC-P

Positive Control

A549 cell lysate, HepG2 cell lysate, K562 cell lysate, human prostate carcinoma tissue.

Concentration

2 mg/mL

Dilution

WB: 1:500-1:1,000 ;IHC-P: 1:50-1:200

Purity

Protein G affinity purified.

Form

Liquid

Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 73 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

No