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Product details

Myoglobin Mouse Monoclonal Antibody [A4B7]

Catalog number EM1902-27

Price for EM1902-27

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Size / count 100 µL

About this product

Myoglobin (symbol Mb or MB) is an iron- and oxygen-binding protein found in the cardiac and skeletal muscle tissue of vertebrates in general and in almost all mammals. Myoglobin is distantly related to hemoglobin. Compared to hemoglobin, myoglobin has a higher affinity for oxygen and does not have cooperative binding with oxygen like hemoglobin does. In humans, myoglobin is only found in the bloodstream after muscle injury. High concentrations of myoglobin in muscle cells allow organisms to hold their breath for a longer period of time. Myoglobin was the first protein to have its three-dimensional structure revealed by X-ray crystallography. Despite being one of the most studied proteins in biology, its physiological function is not yet conclusively established: mice genetically engineered to lack myoglobin can be viable and fertile, but show many cellular and physiological adaptations to overcome the loss. In humans, myoglobin is encoded by the MB gene. Myoglobin can take the forms oxymyoglobin (MbO2), carboxymyoglobin (MbCO), and metmyoglobin (met-Mb), analogously to hemoglobin taking the forms oxyhemoglobin (HbO2), carboxyhemoglobin (HbCO), and methemoglobin (met-Hb).

Specifications

Catalog number
EM1902-27
Size / count
100 µL
Dry ice
No
Hazardous
No
Discontinued
No
CAS Number
9007-83-4
Product Name Alternative
MB antibody MGC13548 antibody MYG_HUMAN antibody Myoglobin antibody
Abbreviation
Myoglobin
Swiss Prot
P02144 Human, P04247 Mouse, Q9QZ76 Rat
Cellular Locus
Cytosol, extracellular exosome.
Host
Mouse
Species Reactivity
Human, Mouse, Rat
Immunogen
Recombinant protein within Human Myoglobin aa 1-140 / 154.
Isotype
IgG1
Conjugation
Non-conjugated
Type
Mouse monoclonal Antibody
Applications
WB, IHC-P, IF-Tissue
Positive Control
Mouse skeletal muscle tissue lysate, rat skeletal muscle tissue lysate, mouse heart tissue lysate, rat heart tissue lysate, rat heart tissue, human fetal skeletal muscle tissue.
Concentration
2 mg/mL
Dilution
WB: 1:1,000-1:5,000 ;IHC-P: 1:100-1:500 ;IF-Tissue: 1:50-1:100
Purity
Protein G affinity purified.
Form
Liquid
Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 17 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody
No

Frequently Asked Questions

What is the catalog number for Myoglobin Mouse Monoclonal Antibody [A4B7]?

The catalog number for Myoglobin Mouse Monoclonal Antibody [A4B7] is EM1902-27.

What sizes are available for Myoglobin Mouse Monoclonal Antibody [A4B7]?

Myoglobin Mouse Monoclonal Antibody [A4B7] is available in the following size(s): 100 µL.

Is Myoglobin Mouse Monoclonal Antibody [A4B7] in stock?

Myoglobin Mouse Monoclonal Antibody [A4B7] is currently made to order / not held in immediate stock. Contact us for lead time.

What is Myoglobin Mouse Monoclonal Antibody [A4B7] used for?

Myoglobin (symbol Mb or MB) is an iron- and oxygen-binding protein found in the cardiac and skeletal muscle tissue of vertebrates in general and in almost all mammals. Myoglobin is distantly related to hemoglobin. Compared to hemoglobin, myoglobin has a higher affinity for oxygen and does not have c

What buffer is this product supplied in?

The formulation or storage buffer is listed on the product datasheet. If you require a carrier-free or custom formulation, contact us before ordering, as this may involve a made-to-order production timeline.

What dilution range is recommended for immunohistochemistry (IHC) with this antibody?

A starting IHC dilution range is listed on the datasheet, but should be optimized for your specific tissue type and staining protocol.

Can I request a Safety Data Sheet (SDS) for this product?

Yes, safety data sheets are available for hazardous or chemically classified products and can be provided on request or downloaded from the product page.

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