PRMT5 Mouse Monoclonal Antibody [7-C2]

About this product

Protein arginine N-methyltransferase 5 is an enzyme that in humans is encoded by the PRMT5 gene. PRMT5 symmetrically dimethylates H2AR3, H4R3, H3R2, and H3R8 in vivo, all of which are linked to a range of transcriptional regulatory events. PRMT5 is a highly conserved arginine methyltransferase that translocated from the cytoplasm to the nucleus at embryonic day ~E8.5, and during preimplantation development at the ~4-cell stage. Model organisms have been used in the study of PRMT5 function. Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion. Twenty five tests were carried out on mutant mice and two significant abnormalities were observed. No homozygous mutant embryos were identified during gestation, and therefore none survived until weaning. The remaining tests were carried out on heterozygous mutant adult mice but no further abnormalities were observed. A conditional allele of Prmt5 in the mouse limb shows that it is essential for maintaining a progenitor population, as conditional mutants have limb defects.

Specifications

Catalog number

EM31103

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

72 kDa ICln binding protein antibody 72 kDa ICln-binding protein antibody ANM5_HUMAN antibody Histone synthetic lethal 7, S. cerevisiae, homolog of antibody Histone-arginine N-methyltransferase PRMT5 antibody HMT1 hnRNP methyltransferase like 5 antibody HOMOLOG OF; SKB1 antibody HRMT1L5 antibody IBP72 antibody Jak-binding protein 1 antibody JBP 1 antibody JBP1 antibody PRMT 5 antibody PRMT5 antibody Protein arginine methyltransferase 5 antibody Protein arginine N methyltransferase 5 antibody Protein arginine N methyltransferase 5 N terminally processed antibody Protein arginine N-methyltransferase 5 antibody S. POMBE antibody S. POMBE HOMOLOG OF; SKB1 antibody SHK1 KINASE BINDING PROTEIN 1 antibody Shk1 kinase binding protein 1 homolog antibody Shk1 kinase-binding protein 1 homolog antibody Shk1 kinase/binding protein 1, S. pombe, homolog of antibody SKB 1 antibody SKB1 antibody SKB1 homolog antibody SKB1: SKB1 homolog (S. pombe) antibody SKB1Hs antibody

Abbreviation

SKB1 homolog,SKB1Hs

Swiss Prot

O14744 Human

Cellular Locus

Nucleus, cytoplasm.

Host

Mouse

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within Human PRMT5 aa 1-50 / 637.

Isotype

IgG2a

Conjugation

Non-conjugated

Type

Mouse monoclonal Antibody

Applications

WB, IHC-P

Positive Control

Human kidney tissue, human liver tissue, mouse kidney tissue, mouse liver tissue, rat kidney tissue, rat liver tissue.

Concentration

2 mg/mL

Dilution

WB: 1:1,000 ;IHC-P: 1:100-1:200

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 73 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

No