AKT1 Mouse Monoclonal Antibody [D9-9-C9] — image 1 of 3

Product details

AKT1 Mouse Monoclonal Antibody [D9-9-C9]

Catalog number EM40507

Price for EM40507

Size / count 100 µL

About this product

The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mice lacking Akt1 display a 25% reduction in body mass, indicating that Akt1 is critical for transmitting growth-promoting signals, most likely via the igf1 receptor. Mice lacking Akt1 are also resistant to cancer: They experience considerable delay in tumor growth initiated by the large T antigen or the Neuoncogene.

Specifications

Catalog number: EM40507
Size / count: 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Product Name Alternative: AKT 1 antibody AKT antibody AKT1 antibody AKT1_HUMAN antibody MGC99656 antibody PKB antibody PKB-ALPHA antibody PRKBA antibody Protein Kinase B Alpha antibody Protein kinase B antibody Proto-oncogene c-Akt antibody RAC Alpha antibody RAC antibody RAC-alpha serine/threonine-protein kinase antibody RAC-PK-alpha antibody
Abbreviation: PKB,PKB alpha
Swiss Prot: P31749 Human, P31750 Mouse, P47196 Rat
Cellular Locus: Cytoplasm, Nucleus, Cell membrane.
Host: Mouse
Species Reactivity: Human, Mouse, Rat
Immunogen: Synthetic peptide within human AKT1 aa 1-80.
Isotype: IgG2b
Conjugation: Non-conjugated
Type: Mouse monoclonal Antibody
Applications: WB, IHC-P
Positive Control: MCF7 cell lysate, A549 cell lysate, Jurkat cell lysate, C2C12 cell lysate, L-929 cell lysate, C6 cell lysate, human breast carcinoma tissue.
Concentration: 2 mg/mL
Dilution: WB: 1:2,000 ;IHC-P: 1:100-1:200
Purity: Protein A affinity purified.
Form: Liquid
Buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight: Predicted band size: 56 kDa
Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody: No

Frequently Asked Questions

What is the catalog number for AKT1 Mouse Monoclonal Antibody [D9-9-C9]?

The catalog number for AKT1 Mouse Monoclonal Antibody [D9-9-C9] is EM40507.

What sizes are available for AKT1 Mouse Monoclonal Antibody [D9-9-C9]?

AKT1 Mouse Monoclonal Antibody [D9-9-C9] is available in the following size(s): 100 µL.

Is AKT1 Mouse Monoclonal Antibody [D9-9-C9] in stock?

AKT1 Mouse Monoclonal Antibody [D9-9-C9] is currently made to order / not held in immediate stock. Contact us for lead time.

What is AKT1 Mouse Monoclonal Antibody [D9-9-C9] used for?

What is the isotype of this antibody?

Isotype information such as IgG1 or IgG2a is listed in the product specifications on the datasheet, relevant for selecting compatible detection reagents.

Can I get a trial-size or discounted first order of this product?

We don’t generally offer free samples, but trial discounts in exchange for feedback and validation results are almost always possible. Contact us with your intended application to arrange one.

Do you offer bulk or custom quantities of this product?

Yes. Ordering two or more units, or combining this with other products, usually qualifies for a discount depending on quantity. Contact our sales team with your required volume for a custom quote.