Glutathione Peroxidase 1 Mouse Monoclonal Antibody [C5-F6]

About this product

GPX1 is ubiquitously expressed in many tissues, where it protects cells from oxidative stress. Within cells, it localizes to the cytoplasm and mitochondria. As a glutathione peroxidase, GPx1 functions in the detoxification of hydrogen peroxide, specifically by catalyzing the reduction of hydrogen peroxide to water. The glutathione peroxidase also catalyzes the reduction of other organic hydroperoxides, such as lipid peroxides, to the corresponding alcohols. GPx1 typically uses glutathione (GSH) as the reductant, but when glutathione synthetase (GSS) is, as in brain mitochondria, γ-glutamylcysteine can serve as the reductant instead. The protein encoded by this gene protects from CD95-induced apoptosis in cultured breast cancer cells and inhibits 5-lipoxygenase in blood cells, and its overexpression delays endothelial cell death and increases resistance to toxic challenges, especially oxidative stress. This protein is one of only a few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by the nonsense (stop) codon TGA.

Specifications

Catalog number

EM40708

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

AL033363 antibody Cellular glutathione peroxidase antibody Glutathione peroxidase 1 antibody Glutathione peroxidase antibody GPx 1 antibody GPx-1 antibody GPX1 antibody GPX1_HUMAN antibody GPXD antibody GSHPx-1 antibody GSHPX1 antibody MGC14399 antibody MGC88245 antibody

Abbreviation

GSHPx-1,GPx-1

Swiss Prot

P07203 Human

Cellular Locus

Cytoplasm.

Host

Mouse

Species Reactivity

Human

Immunogen

Recombinant protein within Human Glutathione Peroxidase 1 aa 25-203 / 203.

Isotype

IgG2b

Conjugation

Non-conjugated

Type

Mouse monoclonal Antibody

Applications

WB, IF-Cell, IHC-P, FC

Positive Control

THP-1 cell lysate, HEK-293 cell lysate, HepG2 cell lysate, SH-SY5Y cell lysate, HT-29 cell lysate, Hela, HepG2, human kidney tissue.

Concentration

2 mg/mL

Dilution

WB: 1:2,000 ;IHC-P: 1:200 ;IF-Cell: 1:200 ;FC: 1:100-1:200

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 22 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

No