CRISPR-Cas9 Mouse Monoclonal Antibody [8-A6-6]

About this product

The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) Type II system is currently the most commonly used RNA-Guided Endonuclease technology for genome engineering. There are two distinct components to this system: (1) a guide RNA and (2) an endonuclease, in this case the CRISPR associated (Cas) nuclease, Cas9. The gRNA/Cas9 complex is recruited to the target sequence by the base-pairing between the gRNA sequence and the complement to the target sequence in the genomic DNA. For successful binding of Cas9, the genomic target sequence must also contain the correct Protospacer Adjacent Motiff (PAM) sequence immediately following the target sequence.

Specifications

Catalog number

EM50804

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

Cas9 antibody CRISPR-associated endonuclease Cas9/Csn1 antibody CRISPR-Cas9/Csn1 antibody csn1 antibody SpyCas9 antibody

Swiss Prot

Q99ZW2 Streptococcus Pyogenes

Host

Mouse

Species Reactivity

Staphylococcus aureus

Immunogen

Recombinant protein within Streptococcus Pyogenes CRISPR-Cas9 aa1-200.

Isotype

IgM

Conjugation

Non-conjugated

Type

Mouse monoclonal Antibody

Applications

WB, IF-Cell

Positive Control

Human 293T cells transfected with CRISPR-Cas9 lysate.

Concentration

2 mg/mL

Dilution

WB: 1:5,000 ;IF-Cell: 1:200-1:1,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

No