ERK1/2 Rabbit Polyclonal Antibody

About this product

Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs, such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines , and research investigators consider it an important target in the diagnosis and treatment of cancer . Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family, as well as Mos and Tpl2/COT. MEK1 and MEK2 are the primary MAPKKs in this pathway.

Specifications

Catalog number

ER131011

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

ERK 1 antibody ERK 2 antibody ERK-2 antibody ERK1 antibody erk1/2 antibody ERK2 antibody ERT1 antibody ERT2 antibody Extracellular signal regulated kinase 1 antibody Extracellular signal-regulated kinase 2 antibody MAP kinase 1 antibody MAP kinase 2 antibody MAP kinase isoform p42 antibody MAP kinase isoform p44 antibody MAPK 1 antibody MAPK 2 antibody MAPK 3 antibody Mapk1 antibody MAPK2 antibody MAPK3 antibody Mitogen-activated protein kinase 1 antibody Mitogen-activated protein kinase 2 antibody MK01_HUMAN antibody p38 antibody p40 antibody p41 antibody p42-MAPK antibody PRKM 2 antibody

Abbreviation

MAP kinase 3, MAPK 3, ERK-1, p44-MAPK, MAP kinase 1, MAPK 1, ERK-2, p42-MAPK

Swiss Prot

P27361 Human, P28482 Human, P63085 Mouse, Q63844 Mouse, P21708 Rat, P63086 Rat

Cellular Locus

Cytoplasm, Nucleus, Cell junction, Membrane.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within human ERK1 aa 306-360.

Isotype

IgG

Conjugation

Non-conjugated

Type

Rabbit polyclonal Antibody

Applications

WB, IF-Cell, IHC-P

Positive Control

HeLa cell lysate, Jurkat cell lysate, Ramos cell lysate, MCF7 cell lysate, Neuro-2a cell lysate, C6 cell lysate, HeLa, RAW264.7, PC-12, human colon carcinoma tissue, human breast carcinoma tissue, mouse large intestine tissue.

Concentration

1 mg/mL

Dilution

WB: 1:2,000-1:5,000 ;IF-Cell: 1:200 ;IHC-P: 1:200

Purity

Immunogen affinity purified.

Form

Liquid

Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 43/41 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

No