iNOS Rabbit Polyclonal Antibody

About this product

Nitric oxide (NO) has a broad range of biological activities and has been implicated in signaling pathways in phylogenetically diverse species. Nitric oxide synthases (NOSs), the enzymes responsible for synthesis of NO, contain an N-terminal oxygenase domain and a C-terminal reductase domain. NOS activity requires homodimerization as well as three cosubstrates (L-arginine, NADPH and O2) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin and heme). Several distinct NOS isoforms have been described and been shown to represent the products of three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS, including NOS1 (also designated ncNOS) whose activity was first identified in neurons, and NOS3 (also designated ecNOS), first identified in endothelial cells. The inducible form of NOS, NOS2 (also designated iNOS), is Ca2+-independent and is expressed in a broad range of cell types.

Specifications

Catalog number

ER1706-89

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

HEP-NOS antibody Hepatocyte NOS antibody HEPNOS antibody inducible antibody Inducible nitric oxide synthase antibody Inducible NO synthase antibody Inducible NOS antibody iNOS antibody MAC NOS antibody Macrophage NOS antibody Nitric oxide synthase 2 inducible antibody Nitric oxide synthase 2 inducible macrophage antibody nitric oxide synthase 2A (inducible, hepatocytes) antibody Nitric oxide synthase antibody Nitric oxide synthase inducible antibody nitric oxide synthase, macrophage antibody NOS 2 antibody NOS antibody Nos II antibody NOS type II antibody nos2 antibody NOS2_HUMAN antibody NOS2A antibody NOS2A, Inducible, Hepatocyte antibody Peptidyl-cysteine S-nitrosylase NOS2 antibody

Abbreviation

HEP-NOS, Inducible NOS, iNOS, NOS2, NOS2A

Swiss Prot

P35228 Human, P29477 Mouse

Cellular Locus

Cytoskeleton, Nucleus, Cytosol.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat, Chicken

Immunogen

Synthetic peptide within human iNOS aa 1,104-1,153 / 1,153.

Isotype

IgG

Conjugation

Non-conjugated

Type

Rabbit polyclonal Antibody

Applications

IF-Cell, FC, WB, IHC-Fr

Positive Control

RAW264.7 treated with 1μg/mL LPS for 24 hours whole cell lysate, RAW264.7 whole cell lysate, HeLa cell lysate, A549 cell lysate, A549, LOVO.

Concentration

1 mg/mL

Dilution

WB: 1:1,000 ;IF-Cell: 1:50-1:200 ;FC: 1:50-1:100 ;IHC-Fr:1:100

Purity

Immunogen affinity purified.

Form

Liquid

Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 131 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

No