Smad4 Rabbit Polyclonal Antibody

About this product

In muscle physiology, plays a central role in the balance between atrophy and hypertrophy. When recruited by MSTN, promotes atrophy response via phosphorylated SMAD2/4. MSTN decrease causes SMAD4 release and subsequent recruitment by the BMP pathway to promote hypertrophy via phosphorylated SMAD1/5/8. Acts synergistically with SMAD1 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression. Binds to SMAD binding elements (SBEs) (5'-GTCT/AGAC-3') within BMP response element (BMPRE) of cardiac activating regions. Common SMAD (co-SMAD) is the coactivator and mediator of signal transduction by TGF-beta (transforming growth factor). Component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling. Promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. Component of the multimeric SMAD3/SMAD4/JUN/FOS complex which forms at the AP1 promoter site; required for synergistic transcriptional activity in response to TGF-beta. May act as a tumor suppressor. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

Specifications

Catalog number

ER1706-90

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

(Small) Mothers Against Decapentaplegic antibody Deleted in Pancreatic Carcinoma 4 antibody Deleted in Pancreatic Carcinoma antibody Deleted in pancreatic carcinoma locus 4 antibody Deletion target in pancreatic carcinoma 4 antibody DPC 4 antibody DPC4 antibody hSMAD4 antibody JIP antibody MAD homolog 4 antibody MAD mothers against decapentaplegic Drosophila homolog 4 antibody MAD mothers against decapentaplegic homolog 4 antibody MADH 4 antibody MADH4 antibody Med antibody Medea antibody Mothers against decapentaplegic homolog 4 antibody Mothers against decapentaplegic, Drosophila, homolog of, 4 antibody Mothers against DPP homolog 4 antibody MYHRS antibody OTTHUMP00000163548 antibody SMA- and MAD-related protein 4 antibody SMAD 4 antibody SMAD family member 4 antibody SMAD mothers against DPP homolog 4 antibody SMAD4 antibody SMAD4_HUMAN antibody

Abbreviation

MAD homolog 4, Mothers against DPP homolog 4, SMAD 4, Smad4, hSMAD4, SMAD4, DPC4, MADH4

Swiss Prot

Q13485 Human, P97471 Mouse, O70437 Rat

Cellular Locus

Cytoplasm, Nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within human Smad4 aa 500-542.

Isotype

IgG

Conjugation

Non-conjugated

Type

Rabbit polyclonal Antibody

Applications

IF-Cell, IHC-P, FC

Positive Control

A549, Hela, SH-SY5Y, rat large intestine tissue, human placenta tissue, mouse small intestine tissue, HUVEC.

Concentration

1 mg/mL

Dilution

IF-Cell: 1:50-1:200;IHC-P: 1:50-1:100;FC: 1:50-1:100

Purity

Immunogen affinity purified.

Form

Liquid

Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 60 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

No