DIAPH3 Rabbit Polyclonal Antibody — image 1 of 3

Product details

DIAPH3 Rabbit Polyclonal Antibody

Catalog number ER1802-54

Price for ER1802-54

Size / count 100 µL

About this product

DIAPH3 (diaphanous homolog 3), also known as DIAP3, DRF3 or mDia2 of mouse origin, is a 1,193 amino acid member of the formin homology protein family and is required for the correct function of various cellular processes. DIAPH3 binds to both Profilin, a protein involved in cell maintenance, and to the GTP-bound form of Rho (Rho-GTP). Binding to both of these proteins allows DIAPH3 to recruit Profilin to the membrane, in a Rho-dependent manner. At the membrane, DIAPH3 promotes Actin polymerization and is required for stress fiber formation, cytokinesis and transcriptional activation of the serum response factor (SRF). DIAPH3 also regulates Actin dynamics by coupling Src tyrosine kinase (c-Src) and Rho during Actin signaling events. DIAPH3 contains one diaph-anous autoregulatory domain (DAD) and one Rho GTPase-binding domain (GBD). When DAD and GBD are intramolecularly bound, the GBD is occupied and DIAPH3 is inactive. Interruption of the DAD-GBD bond allows the GBD to bind to Rho-GTP, thus activating DIAPH3. Seven isoforms of DIAPH3 exist due to alternative splicing events.

Specifications

Catalog number: ER1802-54
Size / count: 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Product Name Alternative: AN antibody AUNA1 antibody Dia2 antibody diap3 antibody DIAP3_HUMAN antibody DIAPH3 antibody Diaphanous homolog 3 (Drosophila) antibody Diaphanous homolog 3 antibody Diaphanous related formin 3 antibody Diaphanous, Drosophila, homolog of, 3 antibody Diaphanous-related formin-3 antibody DKFZp434C0931 antibody DKFZp686A13178 antibody DRF3 antibody FLJ34705 antibody mDia2 antibody NSDAN antibody OTTHUMP00000018480 antibody Protein diaphanous homolog 3 antibody RP11-26P21.1 antibody
Abbreviation: DRF3, DIAPH3, DIAP3
Swiss Prot: Q9NSV4 Human, Q9Z207 Mouse
Cellular Locus: Cytoplasm, Nucleus.
Host: Rabbit
Species Reactivity: Human, Mouse
Immunogen: Synthetic peptide within Human DIAPH3 aa 1,059-1,108 / 1,193.
Isotype: IgG
Conjugation: Non-conjugated
Type: Rabbit polyclonal Antibody
Applications: WB, IF-Cell, IHC-P, FC
Positive Control: A431, LOVO, SiHa, human kidney tissue, human placenta tissue.
Concentration: 1 mg/mL
Dilution: IF-Cell: 1:50-1:200;IHC-P: 1:50-1:400;FC: 1:50-1:100;WB: 1:500
Purity: Immunogen affinity purified.
Form: Liquid
Buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight: Predicted band size: 137/80 kDa
Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody: No

Frequently Asked Questions

What is the catalog number for DIAPH3 Rabbit Polyclonal Antibody?

The catalog number for DIAPH3 Rabbit Polyclonal Antibody is ER1802-54.

What sizes are available for DIAPH3 Rabbit Polyclonal Antibody?

DIAPH3 Rabbit Polyclonal Antibody is available in the following size(s): 100 µL.

Is DIAPH3 Rabbit Polyclonal Antibody in stock?

DIAPH3 Rabbit Polyclonal Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

What is DIAPH3 Rabbit Polyclonal Antibody used for?

Can you help me confirm if this product is right for my specific experiment?

Yes, contact us with details of your sample type, species, and intended application, and our specialists will confirm suitability or suggest an alternative from our supplier network if this isn’t the best fit.

What is the purity of this recombinant protein?

Purity, typically assessed by SDS-PAGE, is listed on the datasheet under quality control data.

What do I do if this product arrives with reduced ice or a damaged cold-chain package?

Report this to us immediately with photos and your order reference. Our specialists will assess whether the product is still usable given the cold-chain interruption, and will arrange a replacement or credit if it isn’t.