BubR1 Rabbit Polyclonal Antibody — image 1 of 3

Product details

BubR1 Rabbit Polyclonal Antibody

Catalog number ER1802-76

Price for ER1802-76

Size / count 100 µL

About this product

Human cells contain two related protein kinases, BUB1 and BUBR1, that appear to have evolved from a single ancestral BUB1 gene. Both kinases are concentrated near the surface of the kinetochore where they monitor kinetochore-microtubule interactions. BUB1 and BUBR1 bind to kinetochores and are postulated to be components of the mitotic checkpoint, which monitors kinetochore activities to determine if chromosomes have achieved alignment at the spindle equator. BUBR1 is essential for normal mitotic progression as it prevents cells from prematurely entering anaphase. BUB3 is a conserved component of the mitotic spindle assembly complex and is also involved with the essential spindle checkpoint pathway that operates during early embryogenesis.

Specifications

Catalog number: ER1802-76
Size / count: 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Product Name Alternative: Beta homolg of S. cerevisiae BUB 1 antibody Beta homolg of S. cerevisiae budding uninhibited by benzimidazoles antibody BUB 1B antibody BUB1 budding uninhibited by benzimidazoles 1 homolog beta antibody Bub1A antibody BUB1B antibody BUB1B_HUMAN antibody BUB1beta antibody BUBR1 antibody Budding Uninhibited by Benzimidazoles 1 beta antibody Budding uninhibited by benzimidazoles 1 homolog beta (yeast) antibody hBUBR1 antibody MAD3/BUB1 related protein kinase antibody MAD3/BUB1-related protein kinase antibody MAD3L antibody Mitotic checkpoint gene BUB1B antibody Mitotic checkpoint kinase MAD3L antibody Mitotic checkpoint serine/threonine protein kinase BUB1 beta antibody Mitotic checkpoint serine/threonine-protein kinase BUB1 beta antibody MVA1 antibody OTTHUMP00000160319 antibody Protein SSK1 antibody SSK 1 antibody SSK1 antibody
Abbreviation: hBUBR1, BUB1B, BUBR1, MAD3L, SSK1
Swiss Prot: O60566 Human
Cellular Locus: Nucleus, Cytoplasm, Cytoskeleton.
Host: Rabbit
Species Reactivity: Human
Immunogen: Recombinant protein within human BubR1 aa 310-620.
Isotype: IgG
Conjugation: Non-conjugated
Type: Rabbit polyclonal Antibody
Applications: WB, IF-Cell, IHC-P, FC
Positive Control: SH-SY-5Y cell lysate, Hela, SH-SY-5Y, SiHa, human spleen tissue, HL-60.
Concentration: 1 mg/mL
Dilution: WB: 1:5,000-1:10,000;IF-Cell: 1:50-1:200;IHC-P: 1:50-1:200;FC: 1:50-1:100
Purity: Immunogen affinity purified.
Form: Liquid
Buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight: Predicted band size: 120 kDa
Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody: No

Frequently Asked Questions

What is the catalog number for BubR1 Rabbit Polyclonal Antibody?

The catalog number for BubR1 Rabbit Polyclonal Antibody is ER1802-76.

What sizes are available for BubR1 Rabbit Polyclonal Antibody?

BubR1 Rabbit Polyclonal Antibody is available in the following size(s): 100 µL.

Is BubR1 Rabbit Polyclonal Antibody in stock?

BubR1 Rabbit Polyclonal Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

What is BubR1 Rabbit Polyclonal Antibody used for?

Is this kit compatible with automated liquid handling platforms?

Automation compatibility varies by kit and isn’t always listed on the datasheet. Contact our technical support team with details of your platform so we can confirm with the manufacturer.

What applications has this product been validated for?

Validated applications such as WB, ELISA, IHC, ICC, or FC are listed on the product datasheet. If you need application-specific validation data before ordering, contact our technical support team with the catalogue number.

How do I reconstitute this lyophilized product?

Reconstitution instructions, including the recommended diluent and final concentration, are provided on the datasheet. Aliquot immediately after reconstitution to avoid repeated freeze-thaw cycles.