NQO1 Rabbit Polyclonal Antibody — image 1 of 3

Product details

NQO1 Rabbit Polyclonal Antibody

Catalog number ER1802-85

Price for ER1802-85

Size / count 100 µL

About this product

NQO1, a 274 amino acid protein, is ubiquitously expressed, but the expression level varies among tissues. NQO1 gene expression is coordinately induced in response to xenobiotics, antioxidants, heavy metals and radiation. The antioxidant response element (ARE) in the NQO1 gene promoter is essential for expression and coordinated induction of NQO1. ARE activation by tert-butylhydroquinone is dependent on PI3-kinase, which lies upstream of Nrf2. Nrf2, c-Jun, Nrf1, Jun-B and Jun-D bind to the ARE and regulate expression and induction of NQO1 gene. Maf-Maf homodimers and possibly Maf-Nrf2 heterodimers play a role in negative regulation of ARE-mediated transcription, but Maf-Nrf1 heterodimers fail to bind with the NQO1 gene ARE and do not repress NQO1 transcription.

Specifications

Catalog number: ER1802-85
Size / count: 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Product Name Alternative: Azoreductase antibody Cytochrome b 5 reductase antibody DHQU antibody DIA 4 antibody DIA4 antibody Diaphorase (NADH/NADPH) (cytochrome b 5 reductase) antibody Diaphorase (NADH/NADPH) antibody Diaphorase 4 antibody Dioxin inducible 1 antibody DT diaphorase antibody DT-diaphorase antibody DTD antibody Menadione reductase antibody NAD (P)H dehydrogenase [quinone] 1 antibody NAD (P)H dehydrogenase quinone 1 antibody NAD (P)H menadione oxidoreductase 1 dioxin inducible antibody NAD (P)H quinone dehydrogenase 1 antibody NAD (P)H: menadione oxidoreductase 1 dioxin inducible 1 antibody NAD (P)H:menadione oxidoreductase 1 antibody NAD (P)H:Quinone acceptor oxidoreductase type 1 antibody NAD (P)H:quinone oxidoreductase 1 antibody NAD (P)H:quinone oxireductase antibody NMOR 1 antibody NMOR I antibody NMOR1 antibody NMORI antibody NQO 1 antibody NQO1 antibody NQO1_HUMAN antibody Phylloquinone reductase antibody QR 1 antibody QR1 antibody Quinone reductase 1 antibody
Abbreviation: DTD, QR1, NQO1, DIA4, NMOR1
Swiss Prot: P15559 Human, Q64669 Mouse, P05982 Rat
Cellular Locus: Cytoplasm.
Host: Rabbit
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant protein within human NQO1 aa 1-270.
Isotype: IgG
Conjugation: Non-conjugated
Type: Rabbit polyclonal Antibody
Applications: WB, FC
Positive Control: Siha cell lysate, A549 cell lysate, Siha cell.
Concentration: 1 mg/mL
Dilution: WB: 1:500-1:1,000 ;FC: 1:50-1:100
Purity: Immunogen affinity purified.
Form: Liquid
Buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight: Predicted band size: 31 kDa
Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody: No

Frequently Asked Questions

What is the catalog number for NQO1 Rabbit Polyclonal Antibody?

The catalog number for NQO1 Rabbit Polyclonal Antibody is ER1802-85.

What sizes are available for NQO1 Rabbit Polyclonal Antibody?

NQO1 Rabbit Polyclonal Antibody is available in the following size(s): 100 µL.

Is NQO1 Rabbit Polyclonal Antibody in stock?

NQO1 Rabbit Polyclonal Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

What is NQO1 Rabbit Polyclonal Antibody used for?

What applications has this product been validated for?

Validated applications such as WB, ELISA, IHC, ICC, or FC are listed on the product datasheet. If you need application-specific validation data before ordering, contact our technical support team with the catalogue number.

How do I reconstitute this lyophilized product?

Reconstitution instructions, including the recommended diluent and final concentration, are provided on the datasheet. Aliquot immediately after reconstitution to avoid repeated freeze-thaw cycles.

What is the recommended incubation time for this antibody?

A suggested incubation time is provided in the recommended protocol on the datasheet, but may need adjusting depending on your sample type and detection system.