sfGFP Rabbit Polyclonal Antibody

About this product

Tagging proteins with a functional sequence is a powerful way to access function, and inserting tags at endogenous genomic loci allows the preservation of a near-native cellular background. GFP11 is the 11th beta-strand of the superfolder GFP beta-barrel structure. When expressed in the same cell, GFP11 and its complementary GFP fragment (GFP1-10) enable functional GFP tagging upon complementation. The key advantage of the GFP11 is its small size (16 aa): this allows commercial ssDNA oligomers to be used as HDR donors, circumventing any requirement for molecular cloning.

Specifications

Catalog number

ER1802-92

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Host

Rabbit

Species Reactivity

Universal

Immunogen

Synthetic peptide immune sequence is RDHMVLHEYVNAAGITC.

Isotype

IgG

Conjugation

Non-conjugated

Type

Rabbit polyclonal Antibody

Applications

WB

Positive Control

GFP11-tagged fusion protein.

Concentration

1 mg/mL

Dilution

WB: 1:1,000

Purity

Immunogen affinity purified.

Form

Liquid

Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

No