PHF8 Rabbit Polyclonal Antibody — image 1 of 3

Product details

PHF8 Rabbit Polyclonal Antibody

Catalog number ER1803-37

Price for ER1803-37

Size / count 100 µL

About this product

Histone lysine demethylase with selectivity for the di- and monomethyl states that plays a key role cell cycle progression, rDNA transcription and brain development. Demethylates mono- and dimethylated histone H3 'Lys-9' residue (H3K9Me1 and H3K9Me2), dimethylated H3 'Lys-27' (H3K27Me2) and monomethylated histone H4 'Lys-20' residue (H4K20Me1). Acts as a transcription activator as H3K9Me1, H3K9Me2, H3K27Me2 and H4K20Me1 are epigenetic repressive marks. Involved in cell cycle progression by being required to control G1-S transition. Required for brain development, probably by regulating expression of neuron-specific genes. Only has activity toward H4K20Me1 when nucleosome is used as a substrate and when not histone octamer is used as substrate. Specifically binds trimethylated 'Lys-4' of histone H3 (H3K4me3), affecting histone demethylase specificity: has weak activity toward H3K9Me2 in absence of H3K4me3, while it has high activity toward H3K9me2 when binding H3K4me3.

Specifications

Catalog number: ER1803-37
Size / count: 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Product Name Alternative: Histone lysine demethylase PHF8 antibody JHDM1F antibody Jumonji C domain containing histone demethylase 1F antibody MRXSSD antibody PHD finger protein 8 antibody PHF8 antibody PHF8_HUMAN antibody ZNF422 antibody
Abbreviation: PHF8, KIAA1111, ZNF422
Swiss Prot: Q9UPP1 Human
Cellular Locus: Nuclear membrane.
Host: Rabbit
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant protein within Human PHF8 aa 153-362 / 1,060.
Isotype: IgG
Conjugation: Non-conjugated
Type: Rabbit polyclonal Antibody
Applications: WB, IF-Cell, IHC-P, FC
Positive Control: PC-3M, A431, Hela, JAR, rat brain tissue , human lung cancer tissue, human colon tissue, mouse brain tissue, human kidney tissue, Siha.
Concentration: 1 mg/mL
Dilution: WB: 1:500-1:2,000;IF-Cell: 1:50-1:200;IHC-P: 1:50-1:200;FC: 1:50-1:100
Purity: Immunogen affinity purified.
Form: Liquid
Buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight: Predicted band size: 118 kDa
Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody: No

Frequently Asked Questions

What is the catalog number for PHF8 Rabbit Polyclonal Antibody?

The catalog number for PHF8 Rabbit Polyclonal Antibody is ER1803-37.

What sizes are available for PHF8 Rabbit Polyclonal Antibody?

PHF8 Rabbit Polyclonal Antibody is available in the following size(s): 100 µL.

Is PHF8 Rabbit Polyclonal Antibody in stock?

PHF8 Rabbit Polyclonal Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

What is PHF8 Rabbit Polyclonal Antibody used for?

What should I do if I need to report a performance issue with a specific lot of this product?

Provide the catalogue number, product name, lot number, and your experimental results to our technical support team. They’ll investigate and, if needed, escalate to the manufacturer, provided the product was used before expiry and stored correctly.

What is the isotype of this antibody?

Isotype information such as IgG1 or IgG2a is listed in the product specifications on the datasheet, relevant for selecting compatible detection reagents.

Can I get a trial-size or discounted first order of this product?

We don’t generally offer free samples, but trial discounts in exchange for feedback and validation results are almost always possible. Contact us with your intended application to arrange one.