MSH2 Rabbit Polyclonal Antibody — image 1 of 2

Product details

MSH2 Rabbit Polyclonal Antibody

Catalog number ER1803-86

Price for ER1803-86

Size / count 100 µL

About this product

Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. Recruits DNA helicase MCM9 to chromatin which unwinds the mismatch containg DNA strand. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.

Specifications

Catalog number: ER1803-86
Size / count: 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Product Name Alternative: BAT26 antibody COCA 1 antibody COCA1 antibody DNA mismatch repair protein Msh2 antibody FCC 1 antibody FCC1 antibody hMSH2 antibody HNPCC 1 antibody HNPCC antibody HNPCC1 antibody LCFS2 antibody MSH 2 antibody Msh2 antibody MSH2_HUMAN antibody MutS homolog 2 antibody MutS homolog 2 colon cancer nonpolyposis type 1 antibody MutS protein homolog 2 antibody
Abbreviation: hMSH2, MSH2.
Swiss Prot: P43246 Human
Cellular Locus: Nucleus, Chromosome.
Host: Rabbit
Species Reactivity: Human
Immunogen: Recombinant protein within Human MSH2 aa 1-150.
Isotype: IgG
Conjugation: Non-conjugated
Type: Rabbit polyclonal Antibody
Applications: WB
Positive Control: K562 cell lysates, Wild-type SCC7 whole cell lysates.
Concentration: 1 mg/mL
Dilution: WB: 1:500-1:1,000
Purity: Immunogen affinity purified.
Form: Liquid
Buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight: 104 kDa
Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody: No

Frequently Asked Questions

What is the catalog number for MSH2 Rabbit Polyclonal Antibody?

The catalog number for MSH2 Rabbit Polyclonal Antibody is ER1803-86.

What sizes are available for MSH2 Rabbit Polyclonal Antibody?

MSH2 Rabbit Polyclonal Antibody is available in the following size(s): 100 µL.

Is MSH2 Rabbit Polyclonal Antibody in stock?

MSH2 Rabbit Polyclonal Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

What is MSH2 Rabbit Polyclonal Antibody used for?

What documentation should I request if this product is used for a regulated or intellectual-property-sensitive application?

If the product is subject to intellectual property protections, we’ll advise on any MTA or usage restrictions before shipping. Let us know your intended end use so we can confirm what’s required upfront.

Is this antibody conjugated?

Conjugation status, such as unconjugated, HRP, FITC, or biotin, is specified in the product name and confirmed on the datasheet.

What is the sensitivity of this ELISA kit?

Assay sensitivity, including the minimum detectable dose, is listed on the datasheet. Kits that are assembled on order typically ship within 2-4 weeks.