MICA + MICB Rabbit Polyclonal Antibody

About this product

MHC class I polypeptide-related sequence A/B (MICA/B) is a cell surface glycoprotein encoded by the MICA/B gene located within MHC locus. MICA and MICB are related to MHC class I and has similar domain structure, which is made up of external α1α2α3 domain, transmembrane segment and C-terminal cytoplasmic tail. MICA/B rather functions as a stress-induced ligand for NKG2D receptor. MICA is broadly recognized by NK cells, γδ T cells, and CD8+ αβ T cells which carry NKG2D receptor on their cell surface. As a result of NKG2D-MICA engagement, effector cytolytic responses of T cells and NK cells against epithelial tumor cells expressing MICA are initiated. MICA/B are not associated with β2-microglobulin nor binds peptides as conventional MHC class I molecules do. The heat shock stress pathway is involved in the regulation of MICA expression as transcription of MICA is regulated by promoter heat shock element.

Specifications

Catalog number

ER1803-95

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

MHC class I chain-related protein A antibody MHC class I chain-related protein B antibody MHC class I polypeptide related sequence A antibody MHC class I polypeptide related sequence B antibody MHC class I polypeptide-related sequence A antibody MIC-A antibody micA antibody MICA_HUMAN antibody MICB antibody

Abbreviation

MIC-A, MIC-B

Swiss Prot

Q29980 Human, Q29983 Human

Cellular Locus

Cell membrane. Cytoplasm.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein within Human MICA + MICB aa 182-325 / 383.

Isotype

IgG

Conjugation

Non-conjugated

Type

Rabbit polyclonal Antibody

Applications

WB, IF-Cell, IHC-P

Positive Control

U937, Mouse bone marrow, A431, rat skin tissue, human lung cancer tissue, human colon tissue, human breast carcinoma tissue, mouse stomach tissue.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:2,000;IF-Cell: 1:50-1:100;IHC-P: 1:50-1:200

Purity

Immunogen affinity purified.

Form

Liquid

Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

42 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

No