Gasdermin D (N terminal) Rabbit Polyclonal Antibody

About this product

Gasdermin-D, N-terminal: Promotes pyroptosis in response to microbial infection and danger signals. Produced by the cleavage of gasdermin-D by inflammatory caspases CASP1 or CASP4 in response to canonical, as well as non-canonical (such as cytosolic LPS) inflammasome activators. After cleavage, moves to the plasma membrane where it strongly binds to inner leaflet lipids, including monophosphorylated phosphatidylinositols, such as phosphatidylinositol 4-phosphate, bisphosphorylated phosphatidylinositols, such as phosphatidylinositol (4,5)-bisphosphate, as well as phosphatidylinositol (3,4,5)-bisphosphate, and more weakly to phosphatidic acid and phosphatidylserine. Homooligomerizes within the membrane and forms pores of 10 - 15 nanometers (nm) of inner diameter, possibly allowing the release of mature IL1B and triggering pyroptosis . Exhibits bactericidal activity. Gasdermin-D, N-terminal released from pyroptotic cells into the extracellular milieu rapidly binds to and kills both Gram-negative and Gram-positive bacteria, without harming neighboring mammalian cells, as it does not disrupt the plasma membrane from the outside due to lipid-binding specificity.

Specifications

Catalog number

ER1901-37

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

1810036L03Rik antibody DF 5L antibody DF5L antibody DFNA 5L antibody DFNA5L antibody FKSG 10 antibody FKSG10 antibody FLJ12150 antibody Gasdermin antibody Gasdermin D antibody Gasdermin domain containing 1 antibody Gasdermin domain containing protein 1 antibody Gasdermin domain-containing protein 1 antibody Gasdermin-D antibody GasderminD antibody GSDMD antibody GSDMD_HUMAN antibody GSDMDC 1 antibody GSDMDC1 antibody

Abbreviation

GSDMD

Swiss Prot

P57764 Human, Q9D8T2 Mouse,

Cellular Locus

Cytosol. Secreted. Plasma membrane.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein within human Gasdermin D aa 100-300.

Isotype

IgG

Conjugation

Non-conjugated

Type

Rabbit polyclonal Antibody

Applications

WB, IHC-P, FC, IF-Cell

Positive Control

SiHa cell lysate, PC-3 cell lysate, Jurkat cell lysate, THP-1 cell lysate, human kidney tissue lysate, SiHa, rat stomach tissue, human tonsil tissue, human prostate carcinoma tissue, human esophagus tissue, mouse colon tissue, mouse intestine tissue, SiHa, PC-3M, RAW264.7, L6.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:2,000 ;IHC-P: 1:50-1:1,000 ;FC: 1:1,000 ;IF-Cell: 1:100

Purity

Immunogen affinity purified.

Form

Liquid

Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 53 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

No