Aldolase B Rabbit Polyclonal Antibody

About this product

Aldolase B also known as fructose-bisphosphate aldolase B or liver-type aldolase is one of three isoenzymes (A, B, and C) of the class I fructose 1,6-bisphosphate aldolase enzyme (EC 4.1.2.13), and plays a key role in both glycolysis and gluconeogenesis. The generic fructose 1,6-bisphosphate aldolase enzyme catalyzes the reversible cleavage of fructose 1,6-bisphosphate (FBP) into glyceraldehyde 3-phosphate and dihydroxyacetone phosphate (DHAP) as well as the reversible cleavage of fructose 1-phosphate (F1P) into glyceraldehyde and dihydroxyacetone phosphate. In mammals, aldolase B is preferentially expressed in the liver, while aldolase A is expressed in muscle and erythrocytes and aldolase C is expressed in the brain. Slight differences in isozyme structure result in different activities for the two substrate molecules: FBP and fructose 1-phosphate. Aldolase B exhibits no preference and thus catalyzes both reactions, while aldolases A and C prefer FBP. In humans, aldolase B is encoded by the ALDOB gene located on chromosome 9. The gene is 14,500 base pairs long and contains 9 exons. Defects in this gene have been identified as the cause of hereditary fructose intolerance (HFI).

Specifications

Catalog number

ER1901-42

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

ALDB antibody ALDO B antibody ALDO2 antibody ALDOB antibody ALDOB_HUMAN antibody Aldolase 2 antibody Aldolase B antibody Aldolase B fructose bisphosphate antibody Aldolase2 antibody AldolaseB antibody EC 4.1.2.13 antibody Fructose bisphosphate aldolase B antibody Fructose-bisphosphate aldolase B antibody Liver type aldolase antibody Liver-type aldolase antibody MS1077 antibody

Abbreviation

ALDOB

Swiss Prot

P05062 Human, Q91Y97 Mouse, P00884 Rat

Cellular Locus

Cytoplasm, Cytoskeleton.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein within Human Aldolase B aa 1-189 / 364.

Isotype

IgG

Conjugation

Non-conjugated

Type

Rabbit polyclonal Antibody

Applications

WB, IHC-P, FC

Positive Control

SH-SY5Y cell lysate, human liver tissue lysate, mouse liver tissue lysate, mouse kidney tissue lysate, rat kidney tissue, human liver tissue, human kidney tissue, mouse small intestine tissue, SH-SY5Y.

Concentration

1 mg/mL

Dilution

WB:1:500-1:2,000 ;IHC-P:1:50-1:200 ;FC:1:50-1:100

Purity

Immunogen affinity purified.

Form

Liquid

Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 39 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

No