Cytokeratin 5+6 Rabbit Polyclonal Antibody

About this product

The protein encoded by this gene is a member of the keratin gene family. Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. Keratin 5 (and K14) are expressed primarily in basal keratinocytes in the epidermis, specifically in the stratified epithelium lining the skin and digestive tract. Keratin intermediate filaments make up the cytoskeletal scaffold within epithelial cells, which contributes to the cell architecture and provides the cells with the ability to withstand mechanical, and non-mechanical, stresses. K5/K14 keratin pairs are able to undergo extensive bundling due to the non-helical tail of K15 acting as a weak cross-linker at the intermediate filament surface. This bundling increases the elasticity, and therefore the mechanical resilience, of the intermediate filaments. K5/K14 intermediate filaments are anchored to the desmosomes of basal cells via desmoplakin and plakophilin-1, connecting the cells to their neighbours. At the hemidesmosome, plectin and BPAG1 associate with transmembrane proteins α6β4 integrin, a type of cell adhesion molecule, and BP180/collagen XVII, linking K5/K14 filaments in the basal cells to the basal lamina. Mutations of the cytokeratin 5 gene (KRT5) have been shown to result in the autosomal dominant disorder epidermolysis bullosa (EB).

Specifications

Catalog number

ER1901-86

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

58 kDa cytokeratin antibody CK-5 antibody CK5 antibody Cytokeratin-5 antibody Cytokeratin5 antibody DDD antibody DDD1 antibody EBS2 antibody epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types antibody K2C5_HUMAN antibody K5 antibody keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types) antibody Keratin 5 antibody Keratin antibody keratin complex 2, basic, gene 5 antibody keratin, type II cytoskeletal 5 antibody Keratin-5 antibody Keratin5 antibody KRT 5 antibody Krt5 antibody KRT5A antibody type II cytoskeletal 5 antibody Type-II keratin Kb5 antibody CK6 antibody CK 6A antibody CK 6B antibody CK 6C antibody CK 6D antibody CK 6E antibody CK-6B antibody CK-6C antibody CK-6E antibody Cytokeratin 6a antibody Cytokeratin 6B antibody Cytokeratin 6C antibody Cytokeratin 6D antibody Cytokeratin 6E antibody Cytokeratin-6B antibody Cytokeratin-6C antibody Cytokeratin-6E antibody K2C6C_HUMAN antibody K6a keratin antibody K6b keratin antibody K6C antibody K6c keratin antibody K6d keratin antibody K6e keratin antibody Keratin antibody Keratin K6h antibody Keratin type II cytoskeletal 6A antibody Keratin type II cytoskeletal 6B antibody Keratin type II cytoskeletal 6C antibody Keratin type II cytoskeletal 6D antibody Keratin type II cytoskeletal 6E antibody Keratin-6C antibody KRT6A antibody KRT6B antibody KRT6C antibody KRT6D antibody KRT6E antibody type II cytoskeletal 6C antibody Type-II keratin Kb12 antibody

Abbreviation

CK-5, K5, KRT5,Keratin, type II cytoskeletal 6A,Cytokeratin-6A,CK-6A,CK-6A,K6A,CK-6D,Cytokeratin-6D,Keratin-6A, K6A,Type-II keratin Kb6,Allergen: Hom s 5,KRT6A

Swiss Prot

P13647 Human, P02538 Human, P04259 Human, Q922U2 Mouse, Q6P6Q2 Rat

Cellular Locus

Cytoskeleton. Cytosol. Nucleus. Extracellular region or secreted.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within Human Cytokeratin 5 aa 187-236 / 590.

Isotype

IgG

Conjugation

Non-conjugated

Type

Rabbit polyclonal Antibody

Applications

WB, IHC-P, FC

Positive Control

A431, human skin tissue, human esophagus tissue, rat skin tissue, mouse skin tissue, human tonsils tissue, Hela.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:1000 ;IHC-P: 1:50-1:600 ;FC: 1;50-1:100

Purity

Immunogen affinity purified.

Form

Liquid

Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 62 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

No