ITCH Rabbit Polyclonal Antibody

About this product

Acts as an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates . Involved in the control of inflammatory signaling pathways . Essential component of a ubiquitin-editing protein complex, comprising also TNFAIP3, TAX1BP1 and RNF11, that ensures the transient nature of inflammatory signaling pathways . Promotes the association of the complex after TNF stimulation . Once the complex is formed, TNFAIP3 deubiquitinates 'Lys-63' polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains . This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NFKB1. Ubiquitinates RIPK2 by 'Lys-63'-linked conjugation and influences NOD2-dependent signal transduction pathways. Regulates the transcriptional activity of several transcription factors, and probably plays an important role in the regulation of immune response . Ubiquitinates NFE2 by 'Lys-63' linkages and is implicated in the control of the development of hematopoietic lineages .

Specifications

Catalog number

ER1901-94

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

ADMFD antibody AIF4 antibody AIP4 antibody Atrophin 1 interacting protein 4 antibody Atrophin-1-interacting protein 4 antibody dJ468O1.1 antibody dJ468O1.1 (atrophin 1 interacting protein 4 (AIP4)) antibody dJ468O1.1 atrophin 1 interacting protein 4 AIP4 antibody E3 ubiquitin protein ligase Itchy homolog antibody E3 ubiquitin-protein ligase Itchy homolog antibody EC 6.3.2 antibody Itch antibody ITCH_HUMAN antibody Itchy E3 ubiquitin protein ligase antibody Itchy E3 ubiquitin protein ligase homolog antibody Itchy E3 ubiquitin protein ligase homolog mouse antibody Itchy E3 ubiquitin protein ligase, mouse, homolog of antibody Itchy homolog E3 ubiquitin protein ligase antibody Itchy mouse homolog E3 ubiquitin protein ligase antibody NAPP1 antibody NFE2 associated polypeptide 1 antibody NFE2-associated polypeptide 1 antibody Ubiquitin protein ligase ITCH antibody

Abbreviation

E3 ubiquitin-protein ligase Itchy homolog ,Itch.Atrophin-1-interacting protein 4,AIP4,NAPP1,ITCH

Swiss Prot

Q96J02 Human, Q8C863 Mouse, A0A8I5ZZU1 Rat

Cellular Locus

Cell membrane, Cytoplasm, Endosome, Membrane, Nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein within Human ITCH aa 302-481 / 903.

Isotype

IgG

Conjugation

Non-conjugated

Type

Rabbit polyclonal Antibody

Applications

WB, IHC-P, IF-Cell, FC

Positive Control

Siha cell lysates, A431 cell lysates, Mouse spleen tissue lysates, Mouse pancreatic tissue lysates, Hela, LOVO, Siha, Human liver cancer tissue, rat brain tissue, mouse pancreas tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1000-1:5000 ;IHC-P: 1:50-1:200 ;IF-Cell: 1:100-1:500 ;FC: 1:50-1:100

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size 102/98/86 kDa.

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

No