MCM7 Rabbit Polyclonal Antibody

About this product

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage. Early fractionation of eukaryotic MCM proteins yielded a variety of dimeric, trimeric and tetrameric complexes with unclear biological significance. Specifically a MCM467 subcomplex is shown to have in vitro helicase activity which is inhibited by the MCM2 subunit. The MCM2-7 hexamer is the proposed physiological active complex.

Specifications

Catalog number

ER1902-60

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

CDABP0042 antibody CDC 47 antibody CDC47 antibody CDC47 homolog antibody Cdc47, S. cerevisiae, homolog of antibody DNA replication licensing factor MCM7 antibody Homolog of S. cerevisiae Cdc47 antibody MCM 2 antibody MCM 7 antibody MCM2 antibody MCM2, formerly antibody Mcm7 antibody MCM7 minichromosome maintenance deficient 7 antibody MCM7_HUMAN antibody Minichromosome Maintainence 7 antibody Minichromosome maintainence, S. cerevisiae, homolog of antibody Minichromosome maintenance complex component 7 antibody Minichromosome maintenance deficient 7 antibody Minichromosome maintenance protein 7 antibody P1.1 MCM3 antibody P1.1-MCM3 antibody P1CDC47 antibody P85MCM antibody PNAS 146 antibody PNAS146 antibody PPP1R104 antibody Protein phosphatase 1, regulatory subunit 104 antibody

Abbreviation

DNA replication licensing factor MCM7 , CDC47 homolog,P1.1-MCM3,MCM7

Swiss Prot

P33993 Human, Q61881 Mouse, Q6AYN8 Rat

Cellular Locus

Nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein corresponding to C terminal Human MCM7.

Isotype

IgG

Conjugation

Non-conjugated

Type

Rabbit polyclonal Antibody

Applications

WB, IHC-P, IF-Cell, FC

Positive Control

MCF-7 cell lysate, K562 cell lysate, mouse colon tissue lysate, MCF-7, MG-63, SHSY5Y, human lung cancer tissue, human colon tissue, mouse skin tissue.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:2,000;IHC-P: 1:50-1:200;IF-Cell: 1:50-1:200;FC: 1:50-1:100

Purity

Immunogen affinity purified.

Form

Liquid

Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size 81/60/45 kDa.

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

No