TTF-1 Rabbit Polyclonal Antibody

About this product

Thyroid transcription factor-1 (TTF-1) is a protein that regulates transcription of genes specific for the thyroid, lung, and diencephalon. It is also known as thyroid specific enhancer binding protein. It is used in anatomic pathology as a marker to determine if a tumor arises from the lung or thyroid. NKX2.1 can be induced by activin A via SMAD2 signaling in a human embryonic stem cell differentiation model. TTF-1 positive cells are found in the lung as type II pneumocytes and club cells. In the thyroid, follicular and parafollicular cells are also positive for TTF-1. For lung cancers, adenocarcinomas are usually positive, while squamous cell carcinomas and large cell carcinomas are rarely positive. Small cell carcinomas (of any primary site) are usually positive. TTF1 is more than merely a clinical marker of lung adenocarcinoma. It plays an active role in sustaining lung cancer cells in view of the experimental observation that it is mutated in lung cancer. It has been observed that a loss of Nkx2-1 allows for deregulation of transcription factors FOXA1/2 (by relaxing histone deacetylation and methylation-mediated repression of Foxa1/2 by Nkx2-1) causing reactivation of an embryonic gastric differentiation program in pulmonary cells. This results in mucinous lung adenocarcinoma, a source of poor clinical outcomes for patients. However others have found that TTF-1 staining is often positive in pulmonary adenocarcinomas, large cell carcinomas, small-cell lung carcinomas, neuroendocrine tumors other than small-cell lung carcinomas and extrapulmonary small-cell carcinomas. It is also positive in thyroid cancers and is used for monitoring for metastasis and recurrence.

Specifications

Catalog number

ER1902-68

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

AV026640 antibody BCH antibody Benign chorea antibody BHC antibody Homeobox protein NK 2 homolog A antibody Homeobox protein NK-2 homolog A antibody Homeobox protein Nkx 2.1 antibody Homeobox protein Nkx-2.1 antibody Homeobox protein Nkx2.1 antibody NK 2 antibody NK 2 homolog A antibody NK2 antibody NK2 homeobox 1 antibody NK2, drosophila, homolog of, A antibody NK2.1, mouse, homolog of antibody Nkx 2 1 antibody NKX 2.1 antibody NKX 2A antibody NKX2 1 antibody Nkx2-1 antibody NKX2.1 antibody NKX21_HUMAN antibody NKX2A antibody T EBP antibody T/EBP antibody TEBP antibody Thyroid nuclear factor 1 antibody Thyroid nuclear factor antibody Thyroid specific enhancer binding protein antibody Thyroid transcription factor 1 antibody Tin man antibody Tinman antibody TITF 1 antibody TITF1 antibody TTF 1 antibody TTF-1 antibody TTF1 antibody

Abbreviation

Homeobox protein Nkx-2.1,Homeobox protein NK-2 homolog A Thyroid nuclear factor 1 Thyroid transcription factor 1 TTF-1 Thyroid-specific enhancer-binding protein T/EBP NKX2-1

Swiss Prot

P43699 Human, P50220 Mouse, P23441 Rat

Cellular Locus

Nucleus

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within Human TTF-1 aa 300-349 / 371.

Isotype

IgG

Conjugation

Non-conjugated

Type

Rabbit polyclonal Antibody

Applications

WB

Positive Control

Mouse lung tissue lysate, rat lung tissue lysate, 293, Hela.

Concentration

1 mg/mL

Dilution

WB: 1:500

Purity

Immunogen affinity purified.

Form

Liquid

Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size 42/39 kDa.

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

No