TRF2 Rabbit Polyclonal Antibody

About this product

Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes. In addition to its telomeric DNA-binding role, required to recruit a number of factors and enzymes required for telomere protection, including the shelterin complex, TERF2IP/RAP1 and DCLRE1B/Apollo. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Together with DCLRE1B/Apollo, plays a key role in telomeric loop (T loop) formation by generating 3' single-stranded overhang at the leading end telomeres: T loops have been proposed to protect chromosome ends from degradation and repair. Required both to recruit DCLRE1B/Apollo to telomeres and activate the exonuclease activity of DCLRE1B/Apollo. Preferentially binds to positive supercoiled DNA. Together with DCLRE1B/Apollo, required to control the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B) needed for telomere replication during fork passage and prevent aberrant telomere topology. Recruits TERF2IP/RAP1 to telomeres, thereby participating in to repressing homology-directed repair (HDR), which can affect telomere length.

Specifications

Catalog number

ER1902-70

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

Telomeric DNA binding protein antibody Telomeric DNA-binding protein antibody Telomeric repeat binding factor 2 antibody Telomeric repeat binding protein 2 antibody Telomeric repeat-binding factor 2 antibody TERF 2 antibody Terf2 antibody TERF2_HUMAN antibody TRBF 2 antibody TRBF2 antibody TRF 2 antibody TRF2 antibody TTAGGG repeat binding factor 2 antibody TTAGGG repeat-binding factor 2 antibody

Abbreviation

TERF2, TRBF2, TRF2

Swiss Prot

Q15554 Human, O35144 Mouse, D3ZJF7 Rat

Cellular Locus

Nucleus, Chromosome, telomere.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein within Human TRF2 aa 124-301 / 542.

Isotype

IgG

Conjugation

Non-conjugated

Type

Rabbit polyclonal Antibody

Applications

WB, IHC-P, FC

Positive Control

A549, LOVO, Siha, rat uterus tissue, human tonsil tissue, mouse testis tissue.

Concentration

1 mg/mL

Dilution

WB:1:500-1:1,000 ;IHC-P:1:50-1:200 ;FC:1:50-1:100

Purity

Immunogen affinity purified.

Form

Liquid

Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 60 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

No