hNaa50p Rabbit Polyclonal Antibody

About this product

N-alpha-acetyltransferase that acetylates the N-terminus of proteins that retain their initiating methionine . Has a broad substrate specificity: able to acetylate the initiator methionine of most peptides, except for those with a proline in second position . Also displays N-epsilon-acetyltransferase activity by mediating acetylation of the side chain of specific lysines on proteins . Autoacetylates in vivo . The relevance of N-epsilon-acetyltransferase activity is however unclear: able to acetylate H4 in vitro, but this result has not been confirmed in vivo . Component of a N-alpha-acetyltransferase complex containing NAA10 and NAA15, but NAA50 does not influence the acetyltransferase activity of NAA10: this multiprotein complex probably constitutes the major contributor for N-terminal acetylation at the ribosome exit tunnel, with NAA10 acetylating all amino termini that are devoid of methionine and NAA50 acetylating other peptides . Required for sister chromatid cohesion during mitosis by promoting binding of CDCA5/sororin to cohesin: may act by counteracting the function of NAA10 .

Specifications

Catalog number

ER2001-66

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

MAK3 antibody FLJ13194 antibody hNAT5 antibody hSAN antibody Mak3 homolog (S. cerevisiae) antibody Mak3 homolog antibody N-acetyltransferase 13 (GCN5-related) antibody N-acetyltransferase 13 antibody N-acetyltransferase 5 antibody N-acetyltransferase san homolog antibody N-alpha-acetyltransferase 50 antibody N-alpha-acetyltransferase 50, NatE catalytic subunit antibody Naa50 antibody NAA50_HUMAN antibody NAT 5 antibody NAT13 antibody NAT5 antibody NatE catalytic subunit antibody San antibody

Abbreviation

N-alpha-acetyltransferase 50Curated hNaa50p2 N-acetyltransferase 13 N-acetyltransferase 51 hNAT51 N-acetyltransferase san homolog1 hSAN1 N-epsilon-acetyltransferase 50Curated NatE catalytic subunit

Swiss Prot

Q9GZZ1 Human, Q6PGB6 Mouse

Cellular Locus

Cytoplasm, Nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within human hNaa50p aa 1-50.

Isotype

IgG

Conjugation

Non-conjugated

Type

Rabbit polyclonal Antibody

Applications

WB, IHC-P, FC

Positive Control

A431 cell lysate, Hela cell lysate, mouse stomach tissue lysate, rat cerebellum tissue lysate, rat brain tissue, human thyroid tissue, rat cerebellum tissue, human skin tissue, human breast carcinoma tissue, Daudi.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:1,000;IHC-P: 1:200-1:1,000;FC: 1:50-1:200

Purity

Immunogen affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 19/9 kDa.

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

No