p70 S6 Kinase beta Rabbit Polyclonal Antibody

About this product

Serine/threonine-protein kinase that acts downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression. Regulates protein synthesis through phosphorylation of EIF4B, RPS6 and EEF2K, and contributes to cell survival by repressing the pro-apoptotic function of BAD. Under conditions of nutrient depletion, the inactive form associates with the EIF3 translation initiation complex. Upon mitogenic stimulation, phosphorylation by the mammalian target of rapamycin complex 1 (mTORC1) leads to dissociation from the EIF3 complex and activation. The active form then phosphorylates and activates several substrates in the pre-initiation complex, including the EIF2B complex and the cap-binding complex component EIF4B. Also controls translation initiation by phosphorylating a negative regulator of EIF4A, PDCD4, targeting it for ubiquitination and subsequent proteolysis. Promotes initiation of the pioneer round of protein synthesis by phosphorylating POLDIP3/SKAR. In response to IGF1, activates translation elongation by phosphorylating EEF2 kinase (EEF2K), which leads to its inhibition and thus activation of EEF2. Also plays a role in feedback regulation of mTORC2 by mTORC1 by phosphorylating RICTOR, resulting in the inhibition of mTORC2 and AKT1 signaling. Mediates cell survival by phosphorylating the pro-apoptotic protein BAD and suppressing its pro-apoptotic function. Phosphorylates mitochondrial URI1 leading to dissociation of a URI1-PPP1CC complex.

Specifications

Catalog number

ER31205

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

70 kDa ribosomal protein S6 kinase 1 antibody KS6B1_HUMAN antibody p70 alpha antibody P70 beta 1 antibody p70 ribosomal S6 kinase alpha antibody p70 ribosomal S6 kinase beta 1 antibody p70 S6 kinase alpha antibody P70 S6 Kinase antibody p70 S6 kinase, alpha 1 antibody p70 S6 kinase, alpha 2 antibody p70 S6K antibody p70 S6K-alpha antibody p70 S6KA antibody p70 (S6K) alpha antibody p70 (S6K)-alpha antibody p70-alpha antibody p70-S6K 1 antibody p70-S6K antibody P70S6K antibody P70S6K1 antibody p70S6Kb antibody PS6K antibody Ribosomal protein S6 kinase 70kDa polypeptide 1 antibody Ribosomal protein S6 kinase beta 1 antibody Ribosomal protein S6 kinase beta-1 antibody Ribosomal protein S6 kinase I antibody RPS6KB1 antibody S6K antibody S6K-beta-1 antibody S6K1 antibody Serine/threonine kinase 14 alpha antibody Serine/threonine-protein kinase 14A antibody STK14A antibody

Abbreviation

S6K-beta-2,S6K2,P70S6K2,p70-S6K 2,SRK,S6K-beta,p70 S6 kinase beta,p70 S6K-beta,p70 S6KB,p70-beta

Swiss Prot

P23443 Human, Q8BSK8 Mouse, P67999 Rat

Cellular Locus

Cytoplasm, Nucleus

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within human p70 S6 Kinase beta aa 411-460.

Isotype

IgG

Conjugation

Non-conjugated

Type

Rabbit polyclonal Antibody

Applications

WB, IF-Cell, IHC-P, FC

Positive Control

HeLa cell lysate, NIH/3T3 cell lysate, C6 cell lysate, HeLa, human pancreas tissue, HepG2.

Concentration

2 mg/mL

Dilution

WB: 1:1,000 ;IF-Cell: 1:200 ;IHC-P: 1:5,000 ;FC: 1:100-1:200

Purity

Immunogen affinity purified.

Form

Liquid

Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 59 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

No