PUMA Rabbit Polyclonal Antibody — image 1 of 3

Product details

PUMA Rabbit Polyclonal Antibody

Catalog number ER31215

Price for ER31215

Size / count 100 µL

About this product

The expression of PUMA is regulated by the tumor suppressor p53. PUMA is involved in p53-dependent and -independent apoptosis induced by a variety of signals, and is regulated by transcription factors, not by post-translational modifications. After activation, PUMA interacts with antiapoptotic Bcl-2 family members, thus freeing Bax and/or Bak which are then able to signal apoptosis to the mitochondria. Following mitochondrial dysfunction, the caspase cascade is activated ultimately leading to cell death. Several studies have shown that PUMA function is affected or absent in cancer cells. Additionally, many human tumors contain p53 mutations, which results in no induction of PUMA, even after DNA damage induced through irradiation or chemotherapy drugs.Other cancers, which exhibit overexpression of antiapotptic Bcl-2 family proteins, counteract and overpower PUMA-induced apoptosis.

Specifications

Catalog number: ER31215
Size / count: 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Product Name Alternative: BBC 3 antibody Bbc3 antibody BBC3_HUMAN antibody BCL 2 binding component 3 antibody Bcl-2-binding component 3 antibody BCL2 binding component 3 antibody JFY 1 antibody JFY-1 antibody JFY1 antibody p53 up regulated modulator of apoptosis antibody p53 up-regulated modulator of apoptosis antibody p53 Upregulated Modulator of Apoptosis antibody PUMA alpha antibody PUMA/JFY1 antibody
Abbreviation: PUMA,BBC3
Swiss Prot: Q9BXH1 Human
Cellular Locus: Mitochondrion.
Host: Rabbit
Species Reactivity: Human, Mouse
Immunogen: Synthetic peptide within C-terminal residues of PUMA.
Isotype: IgG
Conjugation: Non-conjugated
Type: Rabbit polyclonal Antibody
Applications: WB, IF-Cell, IHC-P, FC
Positive Control: Hela cell lysate, Mouse kidney tissue lysate, SKOV-3, Hela, Lovo, human breast cancer tissue, mouse small intestine tissue, Jurkat.
Concentration: 1 mg/mL
Dilution: WB: 1:500-1:1,000 ;IF-Cell: 1:200 ;IHC-P: 1:200 ;FC:1:100
Purity: Immunogen affinity purified.
Form: Liquid
Buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight: Predicted band size: 21 kDa
Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody: No

Frequently Asked Questions

What is the catalog number for PUMA Rabbit Polyclonal Antibody?

The catalog number for PUMA Rabbit Polyclonal Antibody is ER31215.

What sizes are available for PUMA Rabbit Polyclonal Antibody?

PUMA Rabbit Polyclonal Antibody is available in the following size(s): 100 µL.

Is PUMA Rabbit Polyclonal Antibody in stock?

PUMA Rabbit Polyclonal Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

What is PUMA Rabbit Polyclonal Antibody used for?

How many freeze-thaw cycles can this product tolerate?

We recommend aliquoting the product upon receipt to avoid repeated freeze-thaw cycles, which can reduce activity and stability. This is your responsibility once the product has been delivered.

Is antigen retrieval required for IHC with this antibody?

Recommended antigen retrieval methods, where applicable, are listed on the datasheet under the IHC protocol section.

What temperature does this product ship at?

Shipping temperature is chosen based on the product’s stability needs, ranging from ambient to dry ice, and is indicated on the product page. Any related shipping fee is shown clearly in your quotation.