Hsp70 Recombinant Rabbit Monoclonal Antibody [SA0379]

About this product

The 70 kilodalton heat shock proteins (Hsp70s) are a family of conserved ubiquitously expressedheat shock proteins. Proteins with similar structure exist in virtually all living organisms. The Hsp70s are an important part of the cell's machinery for protein folding, and help to protect cells from stress. When not interacting with a substrate peptide, Hsp70 is usually in an ATP bound state. Hsp70 by itself is characterized by a very weak ATPase activity, such that spontaneous hydrolysis will not occur for many minutes. As newly synthesized proteins emerge from the ribosomes, the substrate binding domain of Hsp70 recognizes sequences of hydrophobic amino acid residues, and interacts with them. This spontaneous interaction is reversible, and in the ATP bound state Hsp70 may relatively freely bind and release peptides. However, the presence of a peptide in the binding domain stimulates the ATPase activity of Hsp70, increasing its normally slow rate of ATP hydrolysis.

Specifications

Catalog number

ET1601-11

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

DnaK type molecular chaperone HSP70 1 antibody Epididymis secretory protein Li 103 antibody FLJ54303 antibody FLJ54370 antibody FLJ54392 antibody FLJ54408 antibody FLJ75127 antibody Heat shock 70 kDa protein 1 antibody Heat shock 70 kDa protein 1/2 antibody Heat shock 70 kDa protein 1A/1B antibody Heat shock 70kDa protein 1A antibody Heat shock 70kDa protein 1B antibody Heat shock induced protein antibody HEL S 103 antibody HSP70 1 antibody HSP70 1B antibody HSP70 2 antibody HSP70-1/HSP70-2 antibody HSP70-1A antibody HSP70.1 antibody HSP70.1/HSP70.2 antibody HSP70I antibody HSP71_HUMAN antibody HSP72 antibody HSPA1 antibody HSPA1A antibody HSPA1B antibody

Abbreviation

HSP70-2, HSP70.2

Swiss Prot

P17879 Mouse, Q61696 Mouse, P0DMV9 Human, P0DMV8 Human, Q07439 Rat

Cellular Locus

Cytoplasm

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein within Mouse Hsp70 aa 403-641 / 641.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P, FC

Positive Control

HeLa cell lysate, A549 cell lysate, MCF7 cell lysate, HCT 116 cell lysate, Mouse brain tissue lysate, Mouse testis tissue lysate, Rat testis tissue lysate, NIH/3T3 cell lysate, C2C12 cell lysate, A549, C2C12, NIH/3T3, human breast cancer tissue, human liver cancer tissue, human testis tissue, mouse testis tissue, rat testis tissue, rat prostate tissue, A549.

Concentration

1 mg/mL

Dilution

WB: 1:2,000-1:5,000 ;IF-Cell: 1:100 ;IF-Tissue: 1:50 ;IHC-P: 1:500-1:1,500 ;FC: 1:100

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 70 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes