HMGB1 Recombinant Rabbit Monoclonal Antibody [SA39-03]

About this product

High mobility group box 1 protein, also known as high-mobility group protein 1 (HMG-1) and amphoterin, is a protein that in humans is encoded by the HMGB1 gene. HMG-1 belongs to the high mobility group and contains a HMG-box domain. Like the histones, HMGB1 is among the most important chromatin proteins. In the nucleus HMGB1 interacts with nucleosomes, transcription factors, and histones. This nuclear protein organizes the DNA and regulates transcription. After binding, HMGB1 bends DNA, which facilitates the binding of other proteins. HMGB1 supports transcription of many genes in interactions with many transcription factors. It also interacts with nucleosomes to loosen packed DNA and remodel the chromatin. Contact with core histones changes the structure of nucleosomes. The presence of HMGB1 in the nucleus depends on posttranslational modifications. When the protein is not acetylated, it stays in the nucleus, but hyperacetylation on lysine residues causes it to translocate into the cytosol. HMGB1 has been shown to play an important role in helping the RAG endonuclease form a paired complex during V (D)J recombination.

Specifications

Catalog number

ET1601-2

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

Yes

CAS Number

9000-83-3

Product Name Alternative

Amphoterin antibody Chromosomal protein, nonhistone, HMG1 antibody DKFZp686A04236 antibody High mobility group 1 antibody High mobility group box 1 antibody High mobility group protein 1 antibody High mobility group protein B1 antibody high-mobility group (nonhistone chromosomal) protein 1 antibody HMG-1 antibody HMG1 antibody HMG3 antibody HMGB 1 antibody HMGB1 antibody HMGB1_HUMAN antibody NONHISTONE CHROMOSOMAL PROTEIN HMG1 antibody SBP 1 antibody Sulfoglucuronyl carbohydrate binding protein antibody

Abbreviation

HMG-1

Swiss Prot

P09429 Human, P63158 Mouse, P63159 Rat

Cellular Locus

Cytoplasm, Nucleus, Cell membrane, Secreted, Chromosome

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within Human HMGB1 aa 151-200 / 215.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P, FC

Positive Control

HepG2 cell lysate, HeLa cell lysate, HCT 116 cell lysate, A549 cell lysate, Jurkat cell lysate, C2C12 cell lysate, C6 cell lysate, HeLa, mouse thymus tissue lysate, rat spleen tissue lysate, MCF-7, human tonsil tissue, human kidney tissue, mouse brain tissue, HCT 116, mouse hippocampus tissue.

Concentration

1 mg/mL

Dilution

WB: 1:20,000-1:50,000 ;IF-Cell: 1:500 ;IF-Tissue: 1:100 ;IHC-P: 1:5,000-1:20,000 ;FC: 1:1,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 25 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes