Phospho-Histone H3 (S10) Recombinant Rabbit Monoclonal Antibody [SA31-01]

About this product

Histone H3 is one of the five main histones involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Histone proteins are highly post-translationally modified however Histone H3 is the most extensively modified of the five histones. The term "Histone H3" alone is purposely ambiguous in that it does not distinguish between sequence variants or modification state. Histone H3 is an important protein in the emerging field of epigenetics, where its sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes.

Specifications

Catalog number

ET1601-30

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

H3 3 like sequence MH921 antibody H3 3A antibody H3 a antibody H3 b antibody H3 c antibody H3 d antibody H3 f antibody H3 h antibody H3 histone family member E pseudogene antibody H3 i antibody H3 j antibody H3 k antibody H3 l antibody H33_HUMAN antibody H3F3 antibody H3f3b antibody Histone H3 3 pseudogene antibody Histone H3.3 antibody

Abbreviation

Histone H3.3

Swiss Prot

P68431 Human, P84243 Human, Q16695 Human, Q6NXT2 Human, Q71DI3 Human, P84244 Mouse, P84245 Rat

Cellular Locus

Nucleus, Chromosome

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding Ser10 of human Histone H3.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P, IP, FC, ChIP

Positive Control

HeLa treated with 50nM Calyculin A for 30 minutes whole cell lysate, NIH/3T3 treated with 100nM Calyculin A for 30 minutes whole cell lysate, C6 treated with 100nM Calyculin A for 30 minutes whole cell lysate, HeLa, human kidney tissue, mouse kidney tissue, rat kidney tissue.

Concentration

1 mg/mL

Dilution

WB: 1:2,000-1:10,000 ;IF-Cell: 1:5,000 ;IF-Tissue: 1:50 ;IHC-P: 1:100 ;IP: Use at an assay dependent concentration. ;FC: 1:1,000 ;ChIP: Use 0.5~2 μg for 25 μg of chromatin.

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 15 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes