Cytokeratin 20 Recombinant Rabbit Monoclonal Antibody [SA35-03] — image 1 of 3

Product details

Cytokeratin 20 Recombinant Rabbit Monoclonal Antibody [SA35-03]

Catalog number ET1601-8

Discontinued

Price for ET1601-8

Size / count 100 µL

This variant is discontinued and cannot be added to your cart.

About this product

Keratin 20, often abbreviated CK20, is a protein that in humans is encoded by the KRT20 gene. Keratin 20 is a type I cytokeratin. It is a major cellular protein of mature enterocytes and goblet cells and is specifically found in the gastric and intestinal mucosa. In immunohistochemistry, antibodies to CK20 can be used to identify a range of adenocarcinoma arising from epithelia that normally contain the CK20 protein. For example, the protein is commonly found in colorectal cancer, transitional cell carcinomas and in Merkel cell carcinoma, but is absent in lung cancer, prostate cancer, and non-mucinous ovarian cancer. It is often used in combination with antibodies to CK7 to distinguish different types of glandular tumour.

Specifications

Catalog number: ET1601-8
Size / count: 100 µL
Dry ice: No
Hazardous: No
Discontinued: Yes
CAS Number: 9000-83-3
Product Name Alternative: CD20 antibody CK 20 antibody CK-20 antibody CK20 antibody Cytokeratin-20 antibody Cytokeratin20 antibody K1C20_HUMAN antibody K20 antibody KA20 antibody Keratin 20 antibody keratin 20, type I antibody keratin 21, rat, homolog of antibody Keratin antibody Keratin type I cytoskeletal 20 antibody Keratin-20 antibody Keratin20 antibody KRT 20 antibody KRT 21 antibody KRT20 antibody KRT21 antibody MGC35423 antibody OTTHUMP00000164518 antibody Protein IT antibody type I cytoskeletal 20 antibody
Abbreviation: CK-20, K20
Swiss Prot: P35900 Human, P25030 Rat
Cellular Locus: Cytoplasm
Host: Rabbit
Species Reactivity: Human, Rat
Immunogen: Synthetic peptide within Human Cytokeratin 20 aa 375-424 / 424.
Isotype: IgG
Conjugation: Non-conjugated
Type: Recombinant Rabbit monoclonal Antibody
Applications: WB, IF-Cell, IF-Tissue, IHC-P, FC, IP
Positive Control: HT-29 cell lysate, LoVo cell lysate, CRC cell lysate, CRC, human colon carcinoma tissue, human small intestine tissue, rat small intestine tissue.
Concentration: 1 mg/mL
Dilution: WB: 1:2,000-1:5,000 ;IF-Cell: 1:50 ;IF-Tissue: 1:50 ;IHC-P: 1:50-1:500 ;FC: 1:50 ;IP: Use at an assay dependent concentration.
Purity: Protein A affinity purified.
Form: Liquid
Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight: Predicted band size: 48 kDa
Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody: Yes

Frequently Asked Questions

What is the catalog number for Cytokeratin 20 Recombinant Rabbit Monoclonal Antibody [SA35-03]?

The catalog number for Cytokeratin 20 Recombinant Rabbit Monoclonal Antibody [SA35-03] is ET1601-8.

Is Cytokeratin 20 Recombinant Rabbit Monoclonal Antibody [SA35-03] in stock?

Cytokeratin 20 Recombinant Rabbit Monoclonal Antibody [SA35-03] is currently made to order / not held in immediate stock. Contact us for lead time.

What is Cytokeratin 20 Recombinant Rabbit Monoclonal Antibody [SA35-03] used for?

What dilution range is recommended for immunohistochemistry (IHC) with this antibody?

A starting IHC dilution range is listed on the datasheet, but should be optimized for your specific tissue type and staining protocol.

Can I request a Safety Data Sheet (SDS) for this product?

Yes, safety data sheets are available for hazardous or chemically classified products and can be provided on request or downloaded from the product page.

Can this product be used for co-immunoprecipitation (Co-IP)?

Suitability for Co-IP depends on the specific antibody and is noted under validated applications on the datasheet. If it isn’t listed, contact our technical support team for guidance based on your target and sample type.