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Product details

Phospho-Histone H1.3 (T17)+Histone H1.4 (T17) Recombinant Rabbit Monoclonal Antibody [SR38-03]

Catalog number ET1602-11

Price for ET1602-11

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Size / count 100 µL

About this product

Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene, that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.

Specifications

Catalog number
ET1602-11
Size / count
100 µL
Dry ice
No
Hazardous
No
Discontinued
No
CAS Number
9000-83-3
Product Name Alternative
Histone H1.3 Histone H1c Histone H1s-2 HIST1H1DH1F3 Histone H1.4 Histone H1b Histone H1s-4 HIST1H1EH1F4
Abbreviation
HIST1H1D, HIST1H1E
Swiss Prot
P10412 Human, P16402 Human, P43274 Mouse, P43277 Mouse,
Cellular Locus
Nucleus, Chromosome.
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Thr17 of Human H14.
Isotype
IgG
Conjugation
Non-conjugated
Type
Recombinant Rabbit monoclonal Antibody
Applications
WB, IF-Cell, IF-Tissue, IHC-P
Positive Control
CRC cell lysate, NIH/3T3, CRC, human colon carcinoma tissue, human skin tissue, human breast carcinoma tissue.
Concentration
1 mg/mL
Dilution
WB: 1:500 ;IF-Cell: 1:50 ;IF-Tissue: 1:50 ;IHC-P: 1:200
Purity
Protein A affinity purified.
Form
Liquid
Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 22 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody
Yes

Frequently Asked Questions

What is the catalog number for Phospho-Histone H1.3 (T17)+Histone H1.4 (T17) Recombinant Rabbit Monoclonal Antibody [SR38-03]?

The catalog number for Phospho-Histone H1.3 (T17)+Histone H1.4 (T17) Recombinant Rabbit Monoclonal Antibody [SR38-03] is ET1602-11.

What sizes are available for Phospho-Histone H1.3 (T17)+Histone H1.4 (T17) Recombinant Rabbit Monoclonal Antibody [SR38-03]?

Phospho-Histone H1.3 (T17)+Histone H1.4 (T17) Recombinant Rabbit Monoclonal Antibody [SR38-03] is available in the following size(s): 100 µL.

Is Phospho-Histone H1.3 (T17)+Histone H1.4 (T17) Recombinant Rabbit Monoclonal Antibody [SR38-03] in stock?

Phospho-Histone H1.3 (T17)+Histone H1.4 (T17) Recombinant Rabbit Monoclonal Antibody [SR38-03] is currently made to order / not held in immediate stock. Contact us for lead time.

What is Phospho-Histone H1.3 (T17)+Histone H1.4 (T17) Recombinant Rabbit Monoclonal Antibody [SR38-03] used for?

Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form t

What is the recommended storage temperature after reconstitution?

Post-reconstitution storage guidance is listed on the datasheet, typically requiring aliquoting and storage at -20°C or -80°C to preserve activity.

Can this product be used in multiplex assays?

Multiplex compatibility depends on the specific product and detection panel. Contact our technical support team with details of your multiplex system for guidance before ordering.

What happens if this product doesn’t perform as expected in my experiment?

Send us the catalogue number, product name, lot number, and a summary of your results. Our technical support team will investigate and, where needed, involve the manufacturer. This is covered under our guarantee provided the product was used before its expiry date and stored correctly.

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