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Product details

Phospho-Histone H2A.X (S139) Recombinant Rabbit Monoclonal Antibody [SR33-09]

Catalog number ET1602-2

Discontinued

Price for ET1602-2

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Size / count 100 µL

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About this product

Histone H2A.X is a member of the Histone H2A family, which is involved in nucleosomal organization of chromatin. An important phosphorylated form is γH2AX (S139), which forms when double-strand breaks appear. In humans and other eukaryotes, the DNA is wrapped around histone octamers, consisting of core histones H2A, H2B, H3 and H4, to form chromatin. H2AX contributes to nucleosome-formation, chromatin-remodeling and DNA repair, and is also used in vitro as an assay for double-strand breaks in dsDNA.

Specifications

Catalog number
ET1602-2
Size / count
100 µL
Dry ice
No
Hazardous
No
Discontinued
Yes
CAS Number
9000-83-3
Product Name Alternative
AW228881 antibody H2A histone family member X antibody H2A.FX antibody H2A.X antibody H2a/x antibody H2AFX antibody H2AX antibody H2AX histone antibody H2AX_HUMAN antibody Hist5.2ax antibody Histone 2A antibody Histone 2AX antibody Histone H2A.X antibody Histone H2AX antibody RGD1566119 antibody gamma H2A.X antibody γ H2A.X antibody γ-H2A.X antibody
Abbreviation
H2a/x, gamma H2A.X, H2AX,γ H2A.X,γ-H2A.X
Swiss Prot
P16104 Human, P27661 Mouse
Cellular Locus
Nucleus, Chromosome
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Ser139 of Human Histone H2AX.
Isotype
IgG
Conjugation
Non-conjugated
Type
Recombinant Rabbit monoclonal Antibody
Applications
WB, IHC-P
Positive Control
HeLa treated with 20μM Etoposide for 2 hours whole cell lysate, HeLa treated with UV for 2 hours whole cell lysate, NIH/3T3 treated with 25μM Etoposide for 5 hours whole cell lysate, HepG2 cell lysate–treated with etoposide, rat brain tissue, mouse testis tissue, mouse brain tissue, mouse small intestine tissue, human colon carcinoma tissue.
Concentration
1 mg/mL
Dilution
WB: 1:1,000-1:2,000 ;IHC-P: 1:50-1:5000
Purity
Protein A affinity purified.
Form
Liquid
Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 15 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody
Yes

Frequently Asked Questions

What is the catalog number for Phospho-Histone H2A.X (S139) Recombinant Rabbit Monoclonal Antibody [SR33-09]?

The catalog number for Phospho-Histone H2A.X (S139) Recombinant Rabbit Monoclonal Antibody [SR33-09] is ET1602-2.

Is Phospho-Histone H2A.X (S139) Recombinant Rabbit Monoclonal Antibody [SR33-09] in stock?

Phospho-Histone H2A.X (S139) Recombinant Rabbit Monoclonal Antibody [SR33-09] is currently made to order / not held in immediate stock. Contact us for lead time.

What is Phospho-Histone H2A.X (S139) Recombinant Rabbit Monoclonal Antibody [SR33-09] used for?

Histone H2A.X is a member of the Histone H2A family, which is involved in nucleosomal organization of chromatin. An important phosphorylated form is γH2AX (S139), which forms when double-strand breaks appear. In humans and other eukaryotes, the DNA is wrapped around histone octamers, consisting of c

Is this product suitable for live cell imaging?

Suitability for live cell imaging depends on the specific reagent and conjugate used. Check the datasheet for imaging-specific validation, or contact us for guidance.

Is this product covered by a money-back guarantee?

Yes, we guarantee a full refund or store credit if this product arrives damaged beyond usability, or has a genuine performance issue under proper usage and storage, provided you report it immediately with evidence. Claims after the expiration date or due to improper storage after receipt are excluded.

Can this product be used for diagnostic or therapeutic purposes?

No. This product, like all items we supply, is intended for research laboratory use only and must not be used for diagnostics, therapeutics, treatment, or consumption by humans or animals.

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