FAK Recombinant Rabbit Monoclonal Antibody [SR46-04]

About this product

Focal adhesion kinase was initially identified as a major substrate for the intrinsic protein tyrosine kinase activity of Src encoded pp60. The deduced amino acid sequence of FAK p125 has shown it to be a cytoplasmic protein tyrosine kinase whose sequence and structural organization are unique as compared to other proteins described to date. Localization of p125 by immunofluorescence suggests that it is primarily found in cellular focal adhesions leading to its designation as focal adhesion kinase (FAK). FAK is concentrated at the basal edge of only those basal keratinocytes that are actively migrating and rapidly proliferating in repairing burn wounds and is activated and localized to the focal adhesions of spreading keratinocytes in culture. Thus, it has been postulated that FAK may have an important in vivo role in the reepithelialization of human wounds. FAK protein tyrosine kinase activity has also been shown to increase in cells stimulated to grow by use of mitogenic neuropeptides or neurotransmitters acting through G protein coupled receptors.

Specifications

Catalog number

ET1602-25

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

FADK 1 antibody FADK antibody FAK related non kinase polypeptide antibody FAK1 antibody FAK1_HUMAN antibody Focal adhesion kinase 1 antibody Focal adhesion Kinase antibody Focal adhesion kinase isoform FAK Del33 antibody Focal adhesion kinase related nonkinase antibody FRNK antibody p125FAK antibody pp125FAK antibody PPP1R71 antibody Protein phosphatase 1 regulatory subunit 71 antibody Protein tyrosine kinase 2 antibody Protein-tyrosine kinase 2 antibody Ptk2 antibody PTK2 protein tyrosine kinase 2 antibody

Abbreviation

FADK 1, FRNK, PPP1R71

Swiss Prot

Q05397 Human, P34152 Mouse, O35346 Rat

Cellular Locus

Cytoplasm, Nucleus, Cell membrane, Cell junction.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within human FAK aa 700-740.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P, FC, IP

Positive Control

A431 cell lysate, U-87 MG cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, C6 cell lysate, C2C12, PANC-1, SH-SY5Y, human brain tissue, mouse brain tissue, rat brain tissue, rat kidney tissue, rat spleen tissue.

Concentration

1 mg/mL

Dilution

WB: 1:5,000 ;IF-Cell: 1:50-1:100 ;IF-Tissue: 1:50 ;IHC-P: 1:200-1:1,000 ;FC: 1:50 ;IP: Use at an assay dependent concentration.

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 119 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes